Winkle, Stephen

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Winkle, Stephen

Positions

Associate Professor, Chemistry and Biochemistry , College of Arts, Sciences & Education

winkles@fiu.edu

biography

Research in the Winkle lab focuses on the interactions of small molecules such as antiparasitic agents, carcinogens and anticancer drugs, with DNA and on the interactions of proteins with DNA. We are interested in how these species alter the structure and functioning of DNA and in how altering the structure of the bound molecule affects such alterations. All research in the lab involves undergraduates.

research interests

Biochemistry
Nucleic acid structures
Protein-DNA
Antitumor drug-DNA
Carcinogen-DNA interactions

selected scholarly works & creative activities

Article
- 2024
  
  Effects of thymoquinone binding to DNA on DNA structure and activity. BIOPHYSICAL JOURNAL. 123:81a.
  Full Text via DOI: 10.1016/j.bpj.2023.11.554
- 2014
  
  Probing Sequence and Topological Specificity in the Binding of Tetra(Methylpyridyl)Porphines to DNAs. BIOPHYSICAL JOURNAL. 106:275a.
  Full Text via DOI: 10.1016/j.bpj.2013.11.1611
- 2013
  
  Qualitative analysis of sequence specific binding of flavones to DNA using restriction endonuclease activity assays. BIOPOLYMERS. 99:530-537.
  Full Text via DOI: 10.1002/bip.22212 Web of Science: 000319416400005
- 1992
  
  THE INTERFACE BETWEEN AN ALTERNATING CG MOTIF AND A RANDOM SEQUENCE MOTIF DISPLAYS ALTERED NUCLEASE ACTIVITY. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. 10:389-402.
  Full Text via DOI: 10.1080/07391102.1992.10508654 Web of Science: A1992JZ88800010
- 1992
  
  LOCATING BINDING-SITES FOR THE CARCINOGEN N-ACETOXY-N-ACETYL-2-AMINOFLUORENE USING RESTRICTION ENZYME-INHIBITION ASSAYS. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. 10:83-96.
  Full Text via DOI: 10.1080/07391102.1992.10508632 Web of Science: A1992JL89900007
- 1992
  
  RESTRICTION ENZYME MAPPING OF CARCINOGEN BINDING REGIONS ON PBR322. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. 10:73-82.
  Full Text via DOI: 10.1080/07391102.1992.10508631 Web of Science: A1992JL89900006
- 1992
  
  USING LAMBDA EXONUCLEASE INHIBITION ASSAYS TO MAP CARCINOGEN BINDING-SITES. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. 10:63-72.
  Full Text via DOI: 10.1080/07391102.1992.10508630 Web of Science: A1992JL89900005
- 1991
  
  ENHANCED REACTIVITY OF A B-Z JUNCTION FOR CLEAVAGE BY THE RESTRICTION ENZYME MBOI. BIOCHEMISTRY. 30:10601-10606.
  Full Text via DOI: 10.1021/bi00108a001 Web of Science: A1991GN65800001
- 1990
  
  ANOMALOUS GEL MIGRATION OF DNA OLIGOMERS CONTAINING MULTIPLE CONFORMATIONAL JUNCTIONS. BIOCHEMISTRY. 29:6514-6521.
  Full Text via DOI: 10.1021/bi00479a025 Web of Science: A1990DN23200025
- 1990
  
  Synthesis and characterization of deoxyguanosine–benzoquinone adducts. JOURNAL OF APPLIED TOXICOLOGY. 10:47-54.
  Full Text via DOI: 10.1002/jat.2550100109
- 1989
  
  TEMPERATURE-DEPENDENT CD AND NMR-STUDIES ON A SYNTHETIC OLIGONUCLEOTIDE CONTAINING A B-Z JUNCTION AT HIGH SALT. BIOCHEMISTRY. 28:720-725.
  Full Text via DOI: 10.1021/bi00428a046 Web of Science: A1989T049000046
- 1988
  
  ChemInform Abstract: N‐Methyl‐N′‐oxonicotinium Ion: Synthesis and Stereochemistry.
  Full Text via DOI: 10.1002/chin.198824355
- 1988
  
  Equilibrium binding of spermine and histamine to salmon sperm DNA and poly(dGdC). JOURNAL OF PHARMACY AND PHARMACOLOGY. 40:809-811.
  Full Text via DOI: 10.1111/j.2042-7158.1988.tb05179.x
- 1986
  
  Mixed-mode hydrophobic ion exchange for the separation of oligonucleotides and DNA fragments using HPLC. ANALYTICAL BIOCHEMISTRY. 154:570-577.
  Full Text via DOI: 10.1016/0003-2697(86)90031-X
- 1986
  
  Deoxyguanosine adducts formed from benzoquinone and hydroquinone.
  Full Text via DOI: 10.1007/978-1-4684-5134-4_78
- 1985
  
  ¹³C-NMR of ribosyl A-A-A, A-A-G, AND A-U-G Synthesis and Assignment. BIOPHYSICAL CHEMISTRY. 23:129-138.
  Full Text via DOI: 10.1016/0301-4622(85)80071-5
- 1982
  
  On the cooperative and noncooperative binding of ethidium to DNA. NUCLEIC ACIDS RESEARCH. 10:8211-8223.
  Full Text via DOI: 10.1093/nar/10.24.8211
- 1982
  
  The binding at high base pair/bound drug ratios of small molecules to DNA: Role of side chains in the cooperative binding of intercalators to DNA. BIOPHYSICAL JOURNAL. 37.
- 1981
  
  Equilibrium binding of carcinogens and antitumor antibiotics to DNA: Site selectivity, cooperativity, allosterism. NUCLEIC ACIDS RESEARCH. 9:3175-3186.
  Full Text via DOI: 10.1093/nar/9.13.3175
- 1981
  
  Solute enhanced partition analysis-A novel method for measuring the binding of drugs to DNA. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 98:317-323.
  Full Text via DOI: 10.1016/0006-291X(81)91905-7
- 1979
  
  Interactions of 4-Nitroquinoline 1-Oxide with Deoxyribodinucleotides. BIOCHEMISTRY. 18:3833-3839.
  Full Text via DOI: 10.1021/bi00585a001
- 1978
  
  Interactions of 4-nitroquinoline 1-oxide with four deoxyribonucleotides. BIOCHEMISTRY. 17:1352-1356.
  Full Text via DOI: 10.1021/bi00600a033
- 1976
  
  Modification of fibrosarcomas and squamous cell carcinomas of mouse skin using various tissue extracts. NATURE. 260:48-49.
  Full Text via DOI: 10.1038/260048a0
Book
- 2012
  
  Frontiers in Nucleic Acids
Conference
- 2020
  
  Cooperativity and Competition in the Binding of Heterocyclic Diamidines and RNA Polymerases to phiX174 DNA. BIOPHYSICAL JOURNAL. 222A-222A.
  Web of Science: 000513023201361
- 2017
  
  Effects of heterocyclic diamidine antiparastitic agent binding to DNA on restriction enzyme kinetics. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000430568500872
- 2015
  
  Selectivity, cooperativity, and competition in the binding of heterocyclic diamidines to DNA. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000411183300420
- 2015
  
  Sequence and topological specificity in the binding of tetra(N-methylpyridyl)porphines to DNAs. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000411183300421
- 2014
  
  Probing Sequence and Topological Specificity in the Binding of Tetra(Methylpyridyl) Porphines to DNAs. BIOPHYSICAL JOURNAL. 274A-274A.
  Web of Science: 000337000401510
- 2013
  
  Effects of DNA sequence and topology on the binding of heterocyclic diamidine anti-parasitic agents to DNA examined by restriction enzyme activity assays. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000323851302092
- 2012
  
  Examining the roles of side chains in the sequence selectivity and in the cooperativity of the binding of anthraquinones to DNAs. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000324475100407
- 2009
  
  Restriction enzyme activity studies of the binding of cisplatin to different DNA structures and sequences. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 519-519.
  Web of Science: 000207857800473
- 2008
  
  BIOL 243-RNA polymerase binds to specific nonpromoter DNA sequences. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000271775100603
- 2004
  
  Using restriction enzyme activity assays to investigate the binding of T7 RNA polymerase to DNAs. BIOPHYSICAL JOURNAL. 593A-593A.
  Web of Science: 000187971203059
- 2002
  
  AFLP analysis for the individualization of cannabis sativa.. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. U67-U67.
  Web of Science: 000176296700285
- 2002
  
  The effects of alternate DNA structures on restriction enzyme and polymerase binding and activities with DNAs.. BIOPHYSICAL JOURNAL. 463A-463A.
  Web of Science: 000173252702273
- 2000
  
  Binding of small molecules to the single strand DNA analog of a high affinity carcinogen binding site.. BIOPHYSICAL JOURNAL. 305A-305A.
  Web of Science: 000084779301783
- 1999
  
  Effects of small molecule binding upon the activities of enzymes using a sequence possessing a (CG)(8) segment as a substrate.. BIOPHYSICAL JOURNAL. A131-A131.
  Web of Science: 000081085900764
- 1997
  
  Probing high affinity carcinogen binding DNA sequences with porphyrin.. BIOPHYSICAL JOURNAL. TH439-TH439.
  Web of Science: A1997WE74702417
- 1997
  
  The conformation of (CG) DNA segments regulates RNA polymerase activity.. BIOPHYSICAL JOURNAL. MP424-MP424.
  Web of Science: A1997WE74700559
- 1996
  
  Molecular recognition of unusual DNA structures. BIOPHYSICAL JOURNAL. TU207-TU207.
  Web of Science: A1996TZ68201727
- 1994
  
  WHY CHEMICAL CARCINOGENS BIND SELECTIVELY TO CERTAIN SEQUENCES. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 327-ORGN.
  Web of Science: A1994MY95500404
- 1994
  
  THE INTERACTIONS OF METAL-BOUND INTERCALATORS WITH DNA. BIOPHYSICAL JOURNAL. A185-A185.
  Web of Science: A1994MU46201075
- 1994
  
  WHY CHEMICAL CARCINOGENS BIND SELECTIVELY TO CERTAIN SEQUENCES. BIOPHYSICAL JOURNAL. A231-A231.
  Web of Science: A1994MU46201347
- 1993
  
  RESTRICTION ENZYMES HAVE ALTERED CLEAVAGE RATES AT SITES NEAR CERTAIN SEQUENCES. BIOPHYSICAL JOURNAL. A280-A280.
  Web of Science: A1993KP51701604
- 1992
  
  BINDING OF ANTIBIOTICS TO OLIGONUCLEOTIDES CONTAINING A B-Z JUNCTION. FASEB JOURNAL. A363-A363.
  Web of Science: A1992GY44002077
- 1992
  
  JUNCTIONS AFFECT THE SENSITIVITY OF DNAS TO REACTIONS WITH ENZYMES. FASEB JOURNAL. A219-A219.
  Web of Science: A1992GY44001258
- 1992
  
  SEQUENCE EFFECTS ON HELIX STABILITY AND EASE OF JUNCTION FORMATION IN SYNTHETIC DNA OLIGOMERS CONTAINING B-Z JUNCTIONS. FASEB JOURNAL. A219-A219.
  Web of Science: A1992GY44001256
- 1991
  
  DNA STRUCTURAL PECULIARITIES - CARCINOGEN BINDING-SITES AND B-Z JUNCTIONS. FASEB JOURNAL. A809-A809.
  Web of Science: A1991FC20702545
- 1990
  
  STRUCTURAL FEATURES OF HIGH-AFFINITY CARCINOGEN BINDING-SITES. BIOPHYSICAL JOURNAL. A451-A451.
  Web of Science: A1990CN42801636
- 1990
  
  STRUCTURAL STUDIES OF B-Z JUNCTIONS. BIOPHYSICAL JOURNAL. A450-A450.
  Web of Science: A1990CN42801632
- 1989
  
  H-1-NMR STUDY OF THE TEMPERATURE-DEPENDENCE OF THE CONFORMATION OF A FACE-TO-FACE BIPORPHYRIN. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 198-INOR.
  Web of Science: A1989DG64201713
Note
- 1991
  
  A FURANOCOUMARIN FROM DORSTENIA-CONTRAJERVA. PHYTOCHEMISTRY. 4196-4197.
  Full Text via DOI: 10.1016/0031-9422(91)83504-E Web of Science: A1991GY73700084
Other Scholarly Work
- 2022
  
  Sequence and topological specificity in the binding of thymoquinone to PHIX174RF DNA. BIOPHYSICAL JOURNAL. 63-63.
  Web of Science: 000759523000304
- 2018
  
  Sequence Selectivity, Cooperativity and Competition in the Equilibrium Binding of Psoralens to DNA. BIOPHYSICAL JOURNAL. 596A-596A.
  Full Text via DOI: 10.1016/j.bpj.2017.11.3256 Web of Science: 000430563200735
- 2017
  
  Binding of the Carcinogen 4-Nitroquinoline-1-Oxide to phiX174 DNA - Selectivity and Distortion. BIOPHYSICAL JOURNAL. 213A-214A.
  Full Text via DOI: 10.1016/j.bpj.2016.11.1179 Web of Science: 000402375600067
- 2011
  
  Examining Flanking Sequence Specificity and Topological Specificity in the Binding of Various Molecular Types to DNAs Using Restriction Endonuclease Activity Assays. ACS SYMPOSIUM SERIES. 167-180.
  Web of Science: 000311058100010
- 1986
  
  13C-NMR of ribosyl ApApA, ApApG and ApUpG.. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. 767-781.
  Full Text via DOI: 10.1080/07391102.1986.10508460

chaired theses and dissertations

Aloyo, Maria C., The presence of a non-B-Z-structure enhances an enzyme's reactivity at its recognition site 1992
Bascoy, Marta L., N-acetoxy-N-acetyl-2-aminofluorene binding sites on [phi] X174 and SV40 1991

full name

Stephen Winkle

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publication subject areas

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