Mixed-mode hydrophobic ion exchange for the separation of oligonucleotides and DNA fragments using HPLC Article

Floyd, TR, Cicero, SE, Fazio, SD et al. (1986). Mixed-mode hydrophobic ion exchange for the separation of oligonucleotides and DNA fragments using HPLC . ANALYTICAL BIOCHEMISTRY, 154(2), 570-577. 10.1016/0003-2697(86)90031-X

cited authors

  • Floyd, TR; Cicero, SE; Fazio, SD; Raglione, TV; Hsu, SH; Winkle, SA; Hartwick, RA

authors

abstract

  • Hydrophobic anion exchangers were formed by cobonding both ionic and hydrophobic ligands to silica gel. These phases were used to separate single-stranded oligonucleotides and double-stranded DNA restriction fragments. By varying the ratio of n-octyldimethylsilane and either 3-chloropropyldimethylsilane or 4-chlorobutyldimethylsilane added during silanization a series of mixed-ligand or mixed-mode stationary phases was created. Concentration and ratio of bonded ligands were determined using a new gas chromatography fluorination method. Total ligand coverage was found to approach 2.1 ligands nm2- for n-octyldimethylsilane. Bonding reproducibility for mixed-mode phases was good. Nucleic acid separations were achieved under gentle mobile phase conditions by using the stationary phase as an easily modifiable variable. © 1986.

publication date

  • May 1, 1986

published in

Digital Object Identifier (DOI)

start page

  • 570

end page

  • 577

volume

  • 154

issue

  • 2