On the cooperative and noncooperative binding of ethidium to DNA Article

Winkle, SA, Rosenberg, LS, Krugh, TR. (1982). On the cooperative and noncooperative binding of ethidium to DNA . Nucleic Acids Research (NAR), 10(24), 8211-8223. 10.1093/nar/10.24.8211

cited authors

  • Winkle, SA; Rosenberg, LS; Krugh, TR

abstract

  • The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dc).poly(dG-dC) has been studied by both phase partition and direct spectrophometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E.coli DNA. on the other hand, no evidence of coperative binding was observed in the binding isotherms obtained with calf thymus,C. perfringens, M.Lysodeikticus, or poly(dG-dC). (dG-dC) under the experimental conditions used (0.1 M Nacl). © 1982 IRL Press Limited.

authors

publication date

  • December 20, 1982

published in

Digital Object Identifier (DOI)

start page

  • 8211

end page

  • 8223

volume

  • 10

issue

  • 24