Annamalai, Thirunavukkarasu

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Annamalai, Thirunavukkarasu Graduate Faculty

Positions

Research Assistant Professor, Chemistry and Biochemistry , College of Arts, Sciences & Education

athiruna@fiu.edu

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selected scholarly works & creative activities

Article
- 2026
  
  A high-throughput fluorescent DNA cleavage assay for discovering bacterial DNA gyrase poisons. NUCLEIC ACIDS RESEARCH. 54.
  Full Text via DOI: 10.1093/nar/gkag610 Web of Science: 001794580900001
- 2024
  
  Characterization and antimicrobial activity of endophytic fungi from medicinal plant Agave americana. LETTERS IN APPLIED MICROBIOLOGY. 77.
  Full Text via DOI: 10.1093/lambio/ovae025 Web of Science: 001184795600002
- 2023
  
  Isolation and Characterization of Fungal Endophytes from Petiveria alliacea and Their Antimicrobial Activities in South Florida. Microbiology Research. 14:1470-1482.
  Full Text via DOI: 10.3390/microbiolres14030100 Web of Science: 001073873800001
- 2023
  
  The interaction between transport-segment DNA and topoisomerase IA-crystal structure of MtbTOP1 in complex with both G- and T-segments. NUCLEIC ACIDS RESEARCH. 51:349-364.
  Full Text via DOI: 10.1093/nar/gkac1205 Web of Science: 000905761600001
- 2022
  
  Localization of Mycobacterium tuberculosis topoisomerase I C-terminal sequence motif required for inhibition by endogenous toxin MazF4. FRONTIERS IN MICROBIOLOGY. 13.
  Full Text via DOI: 10.3389/fmicb.2022.1032320 Web of Science: 000899665400001
- 2022
  
  Potent Inhibition of Bacterial DNA Gyrase by Digallic Acid and Other Gallate Derivatives. CHEMMEDCHEM. 17.
  Full Text via DOI: 10.1002/cmdc.202200301 Web of Science: 000870041900001
- 2022
  
  Proton Motive Force-Disrupting Antimycobacterial Guanylurea Polymer. Biomacromolecules. 23:4668-4677.
  Full Text via DOI: 10.1021/acs.biomac.2c00902 Web of Science: 000884681200001
- 2022
  
  A Yeast-Based Screening System for Differential Identification of Poisons and Suppressors of Human Topoisomerase I. Frontiers in Bioscience - Landmark. 27.
  Full Text via DOI: 10.31083/j.fbl2703093 Web of Science: 000790056900022
- 2020
  
  A diverse global fungal library for drug discovery. PEERJ. 8.
  Full Text via DOI: 10.7717/peerj.10392 Web of Science: 000593689800003
- 2020
  
  Mechanistic insights from structure of Mycobacterium smegmatis topoisomerase I with ssDNA bound to both N- and C-terminal domains. NUCLEIC ACIDS RESEARCH. 48:4448-4462.
  Full Text via DOI: 10.1093/nar/gkaa201 Web of Science: 000569096800037
- 2020
  
  Covalent Complex of DNA and Bacterial Topoisomerase: Implications in Antibacterial Drug Development. CHEMMEDCHEM. 15:623-631.
  Full Text via DOI: 10.1002/cmdc.201900721 Web of Science: 000520292700001
- 2020
  
  Inhibition of base excision repair by natamycin suppresses prostate cancer cell proliferation. BIOCHIMIE. 168:241-250.
  Full Text via DOI: 10.1016/j.biochi.2019.11.008 Web of Science: 000503444300022
- 2019
  
  Mechanism and resistance for antimycobacterial activity of a fluoroquinophenoxazine compound. PLOS ONE. 14.
  Full Text via DOI: 10.1371/journal.pone.0207733 Web of Science: 000459709100003
- 2018
  
  Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site. NUCLEIC ACIDS RESEARCH. 46:7296-7308.
  Full Text via DOI: 10.1093/nar/gky492 Web of Science: 000444131400034
- 2018
  
  Synthesis of Antimicrobial Poly(guanylurea)s. Bioconjugate Chemistry. 29:1006-1009.
  Full Text via DOI: 10.1021/acs.bioconjchem.8b00057 Web of Science: 000430641700020
- 2018
  
  A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons. Methods in molecular biology (Clifton, N.J.). 1703:259-268.
  Full Text via DOI: 10.1007/978-1-4939-7459-7_18 Web of Science: 000686716600019
- 2017
  
  Synthesis, evaluation, and CoMFA study of fluoroquinophenoxazine derivatives as bacterial topoisomerase IA inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY. 125:515-527.
  Full Text via DOI: 10.1016/j.ejmech.2016.09.053 Web of Science: 000390496600040
- 2016
  
  Small-Molecule Inhibitors Targeting Topoisomerase I as Novel Antituberculosis Agents. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 60:4028-4036.
  Full Text via DOI: 10.1128/AAC.00288-16 Web of Science: 000378094400020
- 2015
  
  Antimicrobial Susceptibility and SOS-Dependent Increase in Mutation Frequency Are Impacted by Escherichia coli Topoisomerase I C-Terminal Point Mutation. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 59:6195-6202.
  Full Text via DOI: 10.1128/AAC.00855-15 Web of Science: 000367591800041
- 2015
  
  Inhibition of Zn(II) Binding Type IA Topoisomerases by Organomercury Compounds and Hg(II). PLOS ONE. 10.
  Full Text via DOI: 10.1371/journal.pone.0120022 Web of Science: 000351987300103
- 2014
  
  A surface plasmon resonance study of the intermolecular interaction between Escherichia coli topoisomerase I and pBAD/Thio supercoiled plasmid DNA. Biochemical and Biophysical Research Communications. 445:445-450.
  Full Text via DOI: 10.1016/j.bbrc.2014.02.015 Web of Science: 000333228700031
- 2013
  
  Synthesis and antibacterial evaluation of anziaic acid and its analogues as topoisomerase I inhibitors. RSC Medicinal Chemistry. 4:1613-1618.
  Full Text via DOI: 10.1039/c3md00238a Web of Science: 000327461300011
- 2013
  
  Identification of Anziaic Acid, a Lichen Depside from Hypotrachyna sp., as a New Topoisomerase Poison Inhibitor. PLOS ONE. 8.
  Full Text via DOI: 10.1371/journal.pone.0060770 Web of Science: 000317898000064
- 2011
  
  The Strictly Conserved Arg-321 Residue in the Active Site of Escherichia coli Topoisomerase I Plays a Critical Role in DNA Rejoining. Journal of Biological Chemistry. 286:18673-18680.
  Full Text via DOI: 10.1074/jbc.M111.229450 Web of Science: 000290785700040
- 2009
  
  Hydroxyl Radicals Are Involved in Cell Killing by the Bacterial Topoisomerase I Cleavage Complex. JOURNAL OF BACTERIOLOGY. 191:5315-5319.
  Full Text via DOI: 10.1128/JB.00559-09 Web of Science: 000268386600032
- 2009
  
  Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol. BMC Molecular and Cell Biology. 10.
  Full Text via DOI: 10.1186/1471-2091-10-18 Web of Science: 000267522000001
- 2009
  
  Role of proP and proU in betaine uptake by Yersinia enterocolitica under cold and osmotic stress conditions. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 75:1471-1477.
  Full Text via DOI: 10.1128/AEM.01644-08
- 2009
  
  Asp-to-Asn Substitution at the First Position of the DxD TOPRIM Motif of Recombinant Bacterial Topoisomerase I Is Extremely Lethal to E-coli. Journal of Molecular Biology. 385:558-567.
  Full Text via DOI: 10.1016/j.jmb.2008.10.073 Web of Science: 000262916900018
- 2007
  
  Inactivation of Listeria monocytogenes on Frankfurters by monocaprylin alone or in combination with acetic acid. Journal of Food Protection. 70:1594-1599.
  Full Text via DOI: 10.4315/0362-028X-70.7.1594
- 2006
  
  Inactivation of Escherichia coli O157:H7 in cattle drinking water by sodium caprylate. Journal of Food Protection. 69:2248-2252.
  Full Text via DOI: 10.4315/0362-028X-69.9.2248
- 2005
  
  Effect of erythorbate, storage and high-oxygen packaging on premature browning in ground beef. Meat Science. 69:363-369.
  Full Text via DOI: 10.1016/j.meatsci.2004.08.008
- 2005
  
  Expression of major cold shock proteins and genes by Yersinia enterocolitica in synthetic medium and foods. Journal of Food Protection. 68:2454-2458.
  Full Text via DOI: 10.4315/0362-028X-68.11.2454
- 2005
  
  In vitro inactivation of Salmonella enteritidis in autoclaved chicken cecal contents by caprylic acid. Journal of Applied Poultry Research. 14:122-125.
  Full Text via DOI: 10.1093/japr/14.1.122
- 2004
  
  Effect of muscle source on premature browning in ground beef. Meat Science. 68:457-461.
  Full Text via DOI: 10.1016/j.meatsci.2004.04.012
- 2004
  
  In vitro inactivation of Escherichia coli O157:H7 in bovine rumen fluid by caprylic acid. Journal of Food Protection. 67:884-888.
  Full Text via DOI: 10.4315/0362-028X-67.5.884
- 2003
  
  Detection of Penicillium expansum by polymerase chain reaction. International journal of food microbiology. 89:139-144.
  Full Text via DOI: 10.1016/S0168-1605(03)00115-6
- 2003
  
  Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Veterinary Microbiology. 92:179-185.
  Full Text via DOI: 10.1016/S0378-1135(02)00360-7
- 2003
  
  Behavior of enteroaggregative Escherichia coli in bottled spring and mineral water. Journal of Food Protection. 66:497-500.
  Full Text via DOI: 10.4315/0362-028X-66.3.497
- 2003
  
  Heat inactivation of Escherichia coli O157:H7 in apple cider in the presence of glycerol monolaurate and a synthetic antimicrobial peptide, PR-26. Journal of Food Processing and Preservation. 27:37-49.
  Full Text via DOI: 10.1111/j.1745-4549.2003.tb00499.x
- 2001
  
  Inactivation of Escherichia coli O157:H7 and Listeria monocytogenes by PR-26, a synthetic antibacterial peptide. Journal of Food Protection. 64:1929-1934.
  Full Text via DOI: 10.4315/0362-028X-64.12.1929
Conference
- 2021
  
  A DIVERSE GLOBAL FUNGAL LIBRARY FOR DRUG DISCOVERY. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE. 204-204.
  Web of Science: 000778105602327
- 2019
  
  Structure-activity relationship studies of antimicrobial poly-(guanylurea) for overcoming drug resistance. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY.
  Web of Science: 000525061505190
- 2008
  
  Insertions in xanthene dehydrogenase genes suppress cell death due to stabilization of topoisomerase IA covalent complex. FASEB JOURNAL.
  Web of Science: 000208467800226
Other Scholarly Work
- 2026
  
  Discovering Novel DNA Gyrase Poisons using A Unique Fluorescent-based High Throughput Screening Assay. JOURNAL OF BIOLOGICAL CHEMISTRY.
  Full Text via DOI: 10.1016/j.jbc.2026.111903 Web of Science: 001787981600009
- 2025
  
  Bacterial Topoisomerase I Growth Complementation Assay. Methods in molecular biology (Clifton, N.J.). 25-37.
  Full Text via DOI: 10.1007/978-1-0716-4550-5_3
- 2023
  
  Covalent Complex of DNA and Bacterial Topoisomerase: Implications in Antibacterial Drug Development (vol 15, pg 623, 2020). CHEMMEDCHEM.
  Full Text via DOI: 10.1002/cmdc.202100427 Web of Science: 000663166200001
- 2015
  
  Molecular Interaction between Escherichia Coli Topoisomerase I and pBAD/Thio Supercoiled Plasmid DNA. BIOPHYSICAL JOURNAL. 398A-398A.
  Full Text via DOI: 10.1016/j.bpj.2014.11.2181 Web of Science: 000362849400415
Preprint
Review
- 2021
  
  Type IA Topoisomerases as Targets for Infectious Disease Treatments. MICROORGANISMS.
  Full Text via DOI: 10.3390/microorganisms9010086 Web of Science: 000610588900001

principal investigator on

In vitro enzymatic synthesis of circular DNA molecules awarded by NatlCtr Advancing Translational Sciences 2024 - 2026

co-principal investigator on

Grant
- Collaboration for screening of Mycobacterium tuberculosis topoisomerase I inhibitors from DNA-Encoded Library at WuXi awarded by Global Alliance TB for Drug Development 2020 - 2021

investigator on

Structure, Mechanism and Interactions of Type IA Topoisomerases awarded by General Medical Sciences 2021 - 2026

full name

Thirunavukkarasu Annamalai

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Co-investigator Network

publication subject areas

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