FIU Discovery

Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells

Artemisinin and Its Derivate Alleviate Pulmonary Hypertension and Vasoconstriction in Rodent Models.  OXIDATIVE MEDICINE AND CELLULAR LONGEVITY.  2022.

Sodium tanshinone IIA sulfonate enhances the BMP9-BMPR2-Smad1/5/9 signaling pathway in rat pulmonary microvascular endothelial cells and human embryonic stem cell–derived endothelial cells.  BIOCHEMICAL PHARMACOLOGY.  199.

Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary Hypertension.  AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY.  66:452-460.

Established pulmonary hypertension in rats was reversed by a combination of a HIF-2α antagonist and a p53 agonist.  BRITISH JOURNAL OF PHARMACOLOGY.  179:1065-1081.

Mitochondrial Metabolism, Redox, and Calcium Homeostasis in Pulmonary Arterial Hypertension

Endothelial upregulation of mechanosensitive channel Piezo1 in pulmonary hypertension.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  321:C1010-C1027.

Abstract 12319: 16alpha-Hydroxyestrone Downregulates SOX17 During the Development of Pulmonary Arterial Hypertension.  CIRCULATION.  144:a12319-a12319.

Combination Therapy With Rapamycin and Low Dose Imatinib in Pulmonary Hypertension

Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway

Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension.  FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.  9.

Nitration of protein kinase G-Iα modulates cyclic nucleotide crosstalk via phosphodiesterase 3A: Implications for acute lung injury

Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients.  HYPERTENSION.  77:1974-1989.

The mitochondrial redistribution of eNOS is involved in lipopolysaccharide induced inflammasome activation during acute lung injury.  REDOX BIOLOGY.  41.

IL-18 mediates sickle cell cardiomyopathy and ventricular arrhythmias.  BLOOD.  137:1208-1218.

Design and Comprehensive Characterization of Tetramethylpyrazine (TMP) for Targeted Lung Delivery as Inhalation Aerosols in Pulmonary Hypertension (PH): In Vitro Human Lung Cell Culture and In Vivo Efficacy

A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats

Activation of the mechanosensitive Ca²⁺ channel TRPV4 induces endothelial barrier permeability via the disruption of mitochondrial bioenergetics.  REDOX BIOLOGY.  38.

Artemisinin improves acetylcholine-induced vasodilatation in rats with primary hypertension

Cytokines, Chemokines, and Inflammation in Pulmonary Arterial Hypertension

Mitomycin C induces pulmonary vascular endothelial-to-mesenchymal transition and pulmonary veno-occlusive disease via Smad3-dependent pathway in rats.  BRITISH JOURNAL OF PHARMACOLOGY.  178:217-235.

PDGF/MEK/ERK axis represses Ca²⁺clearance via decreasing the abundance of plasma membrane Ca²⁺pump PMCA4 in pulmonary arterial smooth muscle cells.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  320:C66-C79.

RAC1 nitration at Y³² IS involved in the endothelial barrier disruption associated with lipopolysaccharide-mediated acute lung injury.  REDOX BIOLOGY.  38.

Comparison of L-Carnitine and L-Carnitine HCL salt for targeted lung treatment of pulmonary hypertension (PH) as inhalation aerosols: Design, comprehensive characterization, in vitro 2D/3D cell cultures, and in vivo MCT-Rat model of PH.  PULMONARY PHARMACOLOGY & THERAPEUTICS.  65.

Altered Airway Microbiota Composition in Patients With Pulmonary Hypertension.  HYPERTENSION.  76:1589-1599.

A novel rat model of pulmonary hypertension induced by mono treatment with SU5416

Direct extracellular NAMPT involvement in pulmonary hypertension and vascular remodeling transcriptional regulation by SOX and HIF-2a.  AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY.  63:92-103.

Endothelial platelet-derived growth factor-mediated activation of smooth muscle platelet-derived growth factor receptors in pulmonary arterial hypertension.  PULMONARY CIRCULATION.  10.

Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension.  PULMONARY CIRCULATION.  10.

Genetic Admixture and Survival in Diverse Populations with Pulmonary Arterial Hypertension.  AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE.  201:1407-1415.

Tetramethylpyrazine: A promising drug for the treatment of pulmonary hypertension.  BRITISH JOURNAL OF PHARMACOLOGY.  177:2743-2764.

Transcriptomic profiles in pulmonary arterial hypertension associate with disease severity and identify novel candidate genes.  PULMONARY CIRCULATION.  10.

Transplantation of Mesenchymal Stem Cells Attenuates Pulmonary Hypertension by Normalizing the Endothelial-to-Mesenchymal Transition.  AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY.  62:49-60.

Salusin-β Promotes Vascular Calcification via Nicotinamide Adenine Dinucleotide Phosphate/Reactive Oxygen Species-Mediated Klotho Downregulation.  ANTIOXIDANTS & REDOX SIGNALING.  31:1352-1370.

Predicting poor outcomes and the need for surgical treatment in neonates with meconium peritonitis.  PRENATAL DIAGNOSIS.  40:351-357.

Effects of Angiotensin-(1-7) and Angiotensin II on Acetylcholine-Induced Vascular Relaxation in Spontaneously Hypertensive Rats.  OXIDATIVE MEDICINE AND CELLULAR LONGEVITY.  2019.

ALDH2 (Aldehyde Dehydrogenase 2) Protects Against Hypoxia-Induced Pulmonary Hypertension.  ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY.  39:2303-2319.

Angiotensin-(1-7) induced vascular relaxation in spontaneously hypertensive rats.  NITRIC OXIDE-BIOLOGY AND CHEMISTRY.  88:1-9.

Echocardiographic assessment of right ventricular function in experimental pulmonary hypertension.  PULMONARY CIRCULATION.  9.

Genetic determinants of risk in pulmonary arterial hypertension: international genome-wide association studies and meta-analysis

Divergent changes of p53 in pulmonary arterial endothelial and smooth muscle cells involved in the development of pulmonary hypertension.  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY.  316:L216-L228.

Pathogenic Role of mTORC1 and mTORC2 in Pulmonary Hypertension

Establishment and evaluation of chronic obstructive pulmonary disease model by chronic exposure to motor vehicle exhaust combined with lipopolysaccharide instillation

Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension.  CELLULAR SIGNALLING.  51:119-129.

Circulating transcriptome as a signature for the diagnosis of pulmonary arterial hypertension..  FASEB JOURNAL.  32:586.4-586.4.

Endothelial‐dependent activation of smooth muscle PDGF Receptors enhances PASMC proliferation in IPAH.  FASEB JOURNAL.  32:lb444-lb444.

Endothelial HIF-2α contributes to severe pulmonary hypertension due to endothelial-to-mesenchymal transition.  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY.  314:L256-L275.

Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis.  EBIOMEDICINE.  27:18-26.

Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD.  SCIENTIFIC REPORTS.  7.

Chloroquine is a potent pulmonary vasodilator that attenuates hypoxia-induced pulmonary hypertension.  BRITISH JOURNAL OF PHARMACOLOGY.  174:4155-4172.

Expression profile of mitochondrial voltage-dependent anion channel-1 (VDAC1) influenced genes is associated with pulmonary hypertension

Nicotinamide Phosphoribosyltransferase Promotes Pulmonary Vascular Remodeling and Is a Therapeutic Target in Pulmonary Arterial Hypertension.  CIRCULATION.  135:1532-+.

Capsaicin-induced Ca²⁺ signaling is enhanced via upregulated TRPV1 channels in pulmonary artery smooth muscle cells from patients with idiopathic PAH.  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY.  312:1309-1325.

Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats.  CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.  42:2523-2539.

Orai1, 2, 3 and. STIM1 promote store-operated calcium entry in pulmonary arterial smooth muscle cells

miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation.  JOURNAL OF THE AMERICAN HEART ASSOCIATION.  5.

Bortezomib alleviates experimental pulmonary hypertension by regulating intracellular calcium homeostasis in PASMCs.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  311:C482-C497.

Kinematic analysis and experiment of planetary five-bar planting mechanism for zero-speed transplanting on mulch film

Sodium tanshinone IIA sulfonate inhibits hypoxia-induced enhancement of SOCE in pulmonary arterial smooth muscle cells via the PKG-PPAR-γ signaling axis.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  311:C136-C149.

Pathogenic role of calcium-sensing receptors in the development and progression of pulmonary hypertension.  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY.  310:L846-L859.

ID: 59: ROLE OF ENDOTHELIAL HYPOXIA–INDUCIBLE FACTORS IN HYPOXIA-INDUCED PULMONARY VASOCONSTRICITON.  JOURNAL OF INVESTIGATIVE MEDICINE.  64:974.

ID: 72: INHIBITION OF NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE (NAMPT) ATTENUATES EXPERIMENTAL PULMONARY HYPERTENSION.  JOURNAL OF INVESTIGATIVE MEDICINE.  64:912.

ATP promotes cell survival via regulation of cytosolic [Ca²⁺] and Bcl-2/Bax ratio in lung cancer cells.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  310:C99-C114.

Abstract 19126: Endothelial-specific Deletion of Prolyl Hydroxylase Domain Protein 2 (PHD2) Results in Severe Pulmonary Hypertension in Mice by Enhancing the HIF2alpha Signaling Pathway.  CIRCULATION.  132.

Notch Activation of Ca²⁺ Signaling in the Development of Hypoxic Pulmonary Vasoconstriction and Pulmonary Hypertension.  AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY.  53:355-367.

Upregulated expression of STIM2, TRPC6, and Orai2 contributes to the transition of pulmonary arterial smooth muscle cells from a contractile to proliferative phenotype.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  308:C581-C593.

Loss of MicroRNA-17∼92 in Smooth Muscle Cells Attenuates Experimental Pulmonary Hypertension via Induction of PDZ and LIM Domain 5.  AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE.  191:678-692.

HIF2α signaling inhibits adherens junctional disruption in acute lung injury

Deficiency of Akt1, but not Akt2, attenuates the development of pulmonary hypertension.  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY.  308:L208-L220.

Abstract 13284: A Novel Formulation of Vasoactive Intestinal Peptide Attenuates both Acute Hypoxic Pulmonary Vasoconstriction and the Development of Pulmonary Hypertension.  CIRCULATION.  130.

The Sphingosine Kinase 1/Sphingosine-1-Phosphate Pathway in Pulmonary Arterial Hypertension.  AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE.  190:1032-1043.

Flow shear stress enhances intracellular Ca²⁺ signaling in pulmonary artery smooth muscle cells from patients with pulmonary arterial hypertension.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  307:C373-C383.

MiR‐17~92 promotes hypoxia‐induced pulmonary hypertension by regulating proliferation and contraction of pulmonary arterial smooth muscle cells (LB779).  FASEB JOURNAL.  28.

Shear stress enhance intracellular Ca2+ in PASMC from patients with idiopathic pulmonary arterial hypertension (1090.13).  FASEB JOURNAL.  28.

The crucial role of calcium sensing receptor for hypoxia‐induced pulmonary arterial hypertension in mice (889.2).  FASEB JOURNAL.  28.

Upregulated Copper Transporters in Hypoxia-Induced Pulmonary Hypertension.  PLOS ONE.  9.

Adenosine Monophosphate-Activated Protein Kinase Is Required for Pulmonary Artery Smooth Muscle Cell Survival and the Development of Hypoxic Pulmonary Hypertension.  AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY.  49:609-618.

Chronic hypoxia selectively enhances L- and T-type voltage-dependent Ca²⁺ channel activity in pulmonary artery by upregulating Ca_v1.2 and Ca_v3.2.  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY.  305:L154-L164.

Functional characterization of voltage-dependent Ca²⁺ channels in mouse pulmonary arterial smooth muscle cells: divergent effect of ROS.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY.  304:C1042-C1052.

Labeling of Hematopoietic Stem Cells by Tat Peptide Conjugated Quantum Dots for Cell Tracking in Mouse Body.  JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY.  12:6880-6886.

Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases

Akt isoforms differentially regulate neutrophil functions.  BLOOD.  115:4237-4246.

A Novel Function of Sphingosine Kinase 1 Suppression of JNK Activity in Preventing Inflammation and Injury

Applications of Fluorescent Quantum Dots to Stem Cell Tracing In Vivo.  JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY.  9:5726-5730.

Incorporating fluorescent quantum dots into water-soluble polymer

Applications of mesenchymal stem cells labeled with Tat peptide conjugated quantum dots to cell tracking in mouse body.  BIOCONJUGATE CHEMISTRY.  19:421-427.

NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers.  OPTICS EXPRESS.  15:12708-12716.

Inhibition of the activating signals in NK92 cells by recombinant GST-sHLA-G1α chain

Immunoinhibitory effect of soluble HLA-G1 on the cytotoxicity of NK92 cells

Smooth muscle cell ion channels in pulmonary arterial hypertension: Pathogenic role in pulmonary vasoconstriction and vascular remodeling.  295-324.

Deficiency of the Deubiquitinase UCHL1 Attenuates Pulmonary Hypertension.  a1070-a1070.

GALNT1/13 as Novel Mediators of Pulmonary Arterial Hypertension.  a3660-a3660.

The Mitochondrial Redistribution of eNOS is involved in Lipopolysaccharide Induced Inflammasome Activation During Acute Lung Injury.  FREE RADICAL BIOLOGY AND MEDICINE. S101-S101.

Pathogenic Role of Salusin-beta in the Development of Vascular Calcification.  FREE RADICAL BIOLOGY AND MEDICINE. S113-S113.

Preventing RhoA Nitration Attenuates NF-?b Dependent Inflammatory Signaling.  FREE RADICAL BIOLOGY AND MEDICINE. S127-S127.

Circulating transcriptome as a signature for the diagnosis of pulmonary arterial hypertension.  FASEB JOURNAL.

Endothelial-dependent activation of smooth muscle PDGF Receptors enhances PASMC proliferation in IPAH.  FASEB JOURNAL.

Deletion of Rictor in Smooth Muscle Cells Negligibly Affects the Development of Pulmonary Hypertension.  CIRCULATION.

Pathogenic Role of p53 in the Development and Progression of Pulmonary Hypertension.  CIRCULATION.

Capsaicin-sensitive TRPV1 Currents Are Enhanced in Pulmonary Artery Smooth Muscle Cells from Patients with Pulmonary Arterial Hypertension.  FASEB JOURNAL.

Inhibition of PHD2 Induces Endothelial-to-Mesenchymal Transition in Pulmonary Vascular Endothelial Cells.  FASEB JOURNAL.

Activation of the Calcium Sensing Receptor potentially inhibits Kv1.5 channel activity.  FASEB JOURNAL.

Calcium-sensing Receptor, Coupled with Transient Receptor Potential Canonical Channels 6, Regulates Cytosolic [Ca2+] in the Development and Progression of Pulmonary Hypertension.  FASEB JOURNAL.

Hot, Heat and Stretch All Contribute to the Enhanced Ca2+ Signaling in Pulmonary Arterial Hypertension.  FASEB JOURNAL.

PATHOGENIC ROLES OF CALCIUM-SENSING RECEPTORS AND TRANSIENT RECEPTOR POTENTIAL CANONICAL CHANNELS 6 IN THE DEVELOPMENT AND PROGRESSION OF PULMONARY HYPERTENSION.  JOURNAL OF INVESTIGATIVE MEDICINE.

Targeting PTEN/Akt/mTOR signaling pathway in pulmonary arterial hypertension.  S61-S61.

A Novel Formulation of Vasoactive Intestinal Peptide Attenuates both Acute Hypoxic Pulmonary Vasoconstriction and the Development of Pulmonary Hypertension.  CIRCULATION.

MiR-17 similar to 92 promotes hypoxia-induced pulmonary hypertension by regulating proliferation and contraction of pulmonary arterial smooth muscle cells.  FASEB JOURNAL.

Shear stress enhance intracellular Ca2+ in PASMC from patients with idiopathic pulmonary arterial hypertension.  FASEB JOURNAL.

The crucial role of calcium sensing receptor for hypoxia-induced pulmonary arterial hypertension in mice.  FASEB JOURNAL.

Distinct Roles of Akt Isoforms in the Development of Hypoxia Induced Pulmonary Hypertension.  CIRCULATION.

ACTIVATION OF AKT/MTOR PATHWAY CONTRIBUTES TO ENHANCED PULMONARY ARTERY SMOOTH MUSCLE CELL PROLIFERATION IN PATIENTS AND ANIMALS WITH PULMONARY ARTERIAL HYPERTENSION.  JOURNAL OF INVESTIGATIVE MEDICINE. 780-780.

Nonmuscle Myosin Light-Chain Kinase Regulates Neutrophil Oxidant Production during LPS Induced Acute Lung Injury..  AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE.

Editorial: Mitochondrial Metabolism in Ischemic Heart Disease

Editorial: Hypertension: Novel Mechanisms of Nervous and Humoral Regulation

MiRNA208/Mef2 and TNF-α in right ventricular dysfunction: The transition from hypertrophy to failure.  CIRCULATION RESEARCH. 6-8.

RNA Modification Signature of Peripheral Blood as a Potential Diagnostic Marker for Pulmonary Hypertension.  HYPERTENSION. E67-E69.

Editorial: Pathophysiology and Pathogenic Mechanisms of Pulmonary Vascular Disease

Mitochondrial Fission Drives A Glycolytic Endothelial Phenotype In Pulmonary Hypertension: Role Of Nitrated RhoA.  FREE RADICAL BIOLOGY AND MEDICINE. 49-49.

Nitration-Mediated PKG-1alpha Inhibition Disrupts Proteasome-dependent Degradation of PDE3A.  FREE RADICAL BIOLOGY AND MEDICINE. 57-57.

Role Of Mitochondrial ROS and HIF Signaling in the Metabolic Reprogramming Associated with Increased Sox18 Expression.  FREE RADICAL BIOLOGY AND MEDICINE. 56-57.

Corrigendum: Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway (Front. Cell Dev. Biol., (2021), 9, (722365), 10.3389/fcell.2021.722365)

Is p38 MAPK a Dark Force in Right Ventricular Hypertrophy and Failure in Pulmonary Arterial Hypertension?.  AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY. 506-508.

Pathogenic role of ion channels in pulmonary arterial hypertension.  1075-1077.

Targeting L-arginine-nitric oxide-cGMP pathway in pulmonary arterial hypertension.  PULMONARY CIRCULATION. 569-571.

Genetic Insights into Pulmonary Arterial Hypertension Application of Whole-Exome Sequencing to the Study of Pathogenic Mechanisms.  AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE. 393-397.

Gasping for answers. Focus on "Calpain activation by ROS mediates human ether-a-go-go-related gene protein degradation by intermittent hypoxia".  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY. C432-C433.

New insights into the pathology of pulmonary hypertension: implication of the miR-210/ISCU1/2/Fe-S axis.  689-691.

Raptor and Rictor Both Contribute to the Development and Progression of Pulmonary Arterial Hypertension.  FASEB JOURNAL.

The Functional Coupling of CaSR and TRPC6 Contributes to Enhanced Proliferation in Pulmonary Arterial Smooth Muscle Cells.  FASEB JOURNAL.

HIF2 alpha signaling inhibits adherens junctional disruption in acute lung injury (vol 125, pg 652, 2015).  1364-1364.

Transient receptor potential channels (TRPC) contribute to an enhanced endothelial cell proliferation and irreversible vascular remodeling associated with the development of pulmonary arterial hypertension (PAH).  FASEB JOURNAL.

Upregulation of Mechanosensitive Channel Piezo1 Involved in High Shear Stress-induced Pulmonary Hypertension

Research Progress on Pulmonary Arterial Hypertension and the Role of the Angiotensin Converting Enzyme 2-Angiotensin-(1–7)-Mas Axis in Pulmonary Arterial Hypertension.  363-370.

Sex Differences, Estrogen Metabolism and Signaling in the Development of Pulmonary Arterial Hypertension.  FRONTIERS IN CARDIOVASCULAR MEDICINE.

Calcium-Sensing Receptor Regulates Cytosolic [Ca²⁺] and Plays a Major Role in the Development of Pulmonary Hypertension.  FRONTIERS IN PHYSIOLOGY.

A process-based review of mouse models of pulmonary hypertension.  PULMONARY CIRCULATION. 415-433.