Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary Hypertension Article

Huang, Y, Lin, F, Tang, R et al. (2022). Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary Hypertension . AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 66(4), 452-460. 10.1165/rcmb.2021-0414OC

cited authors

  • Huang, Y; Lin, F; Tang, R; Bao, C; Zhou, Q; Ye, K; Shen, Y; Liu, C; Hong, C; Yang, K; Tang, H; Wang, J; Lu, W; Wang, T

authors

abstract

  • in cytokine and chemokine signaling. Protein–protein interaction analysis of the differentially expressed transcripts regulated by DMB showed five hub genes with a strong connectivity of proinflammatory cytokines and chemokines, including Kng1, Cxcl1, Cxcl2, Cxcl6, and Il6. In vitro, TMAO significantly increased the expression of Kng1, Cxcl1, Cxcl2, Cxcl6, and Il6 in bone-marrow-derived macrophage. Also, TMAO-treated conditioned medium from macrophage increased the proliferation and migration of pulmonary artery smooth muscle cells, but TMAO treatment did not change the proliferation or migration of pulmonary artery smooth muscle cells. In conclusion, our study demonstrates that TMAO is increased in severe PH, and the reduction of TMAO decreases pulmonary vascular muscularization and alleviates PH by suppressing the macrophage production of chemokines and cytokines.

publication date

  • April 1, 2022

published in

Digital Object Identifier (DOI)

start page

  • 452

end page

  • 460

volume

  • 66

issue

  • 4