Hackett, John

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Hackett, John Graduate Faculty

Positions

Professor, Chemistry and Biochemistry , College of Arts, Sciences & Education

jhackett@fiu.edu

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biography

John Hackett is a Professor in Chemistry and Biochemistry.

research interests

Skills and Expertise
Molecular Dynamics Simulation
Computational Chemistry
Density Functional Theory
Quantum Chemistry
Estrogens
Cytochrome P450
Spectroscopy
Drug Metabolic Detoxication

selected scholarly works & creative activities

Article
- 2024
  
  Small angle scattering reveals the orientation of cytochrome P450 19A1 in lipoprotein nanodiscs. JOURNAL OF INORGANIC BIOCHEMISTRY. 257.
  Full Text via DOI: 10.1016/j.jinorgbio.2024.112579 Web of Science: 001238690000001
- 2024
  
  HCV Antiviral Drugs Have the Potential to Adversely Perturb the Fetal-Maternal Communication Axis through Inhibition of CYP3A7 DHEA-S Oxidation
  Full Text via DOI: 10.1124/dmd.123.001434 Web of Science: 001248461100007
- 2023
  
  Induced Fit Describes Ligand Binding to Membrane-Associated Cytochrome P450 3A4
  Full Text via DOI: 10.1124/molpharm.123.000698 Web of Science: 001085385800003
- 2023
  
  Nanodisc-embedded cytochrome P450 P3A4 binds diverse ligands by distributing conformational dynamics to its flexible elements. JOURNAL OF INORGANIC BIOCHEMISTRY. 244.
  Full Text via DOI: 10.1016/j.jinorgbio.2023.112211 Web of Science: 000987193200001
- 2023
  
  Low molecular weight ligands bind to CYP3A4 via a branched induced fit mechanism: Implications for O2 binding. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 739.
  Full Text via DOI: 10.1016/j.abb.2023.109582 Web of Science: 000965224000001
- 2022
  
  Structures and Dynamics of Anionic Lipoprotein Nanodiscs. JOURNAL OF PHYSICAL CHEMISTRY B. 126:2850-2862.
  Full Text via DOI: 10.1021/acs.jpcb.2c00758 Web of Science: 000790816100007
- 2020
  
  Dynamics and Mechanism of Binding of Androstenedione to Membrane-Associated Aromatase. BIOCHEMISTRY. 59:2999-3009.
  Full Text via DOI: 10.1021/acs.biochem.0c00460 Web of Science: 000566337500001
- 2020
  
  Conformational selection is present in ligand binding to cytochrome P450 19A1 lipoprotein nanodiscs. JOURNAL OF INORGANIC BIOCHEMISTRY. 209.
  Full Text via DOI: 10.1016/j.jinorgbio.2020.111120
- 2020
  
  Dynamics and Location of the Allosteric Midazolam Site in Cytochrome P4503A4 in Lipid Nanodiscs. BIOCHEMISTRY. 59:766-779.
  Full Text via DOI: 10.1021/acs.biochem.9b01001
- 2018
  
  Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
  Full Text via DOI: 10.1074/jbc.RA118.004044 Web of Science: 000439449700014
- 2018
  
  Biophysical characterization of Aptenodytes forsteri cytochrome P450 aromatase. JOURNAL OF INORGANIC BIOCHEMISTRY. 184:79-87.
  Full Text via DOI: 10.1016/j.jinorgbio.2018.04.002 Web of Science: 000434493300010
- 2018
  
  Biophysical Characterization of Aptenodytes forsteri Cytochrome P450 19 (Aromatase). FASEB JOURNAL. 32:796.22-796.22.
  Full Text via DOI: 10.1096/fasebj.2018.32.1_supplement.796.22
- 2018
  
  Membrane-embedded substrate recognition by cytochrome P450 3A4
  Full Text via DOI: 10.1074/jbc.RA117.000961 Web of Science: 000427587500016
- 2017
  
  Engineered P450 biocatalysts show improved activity and regio-promiscuity in aromatic nitration. SCIENTIFIC REPORTS. 7.
  Full Text via DOI: 10.1038/s41598-017-00897-z Web of Science: 000399186000006
- 2016
  
  N-Heterocyclic Carbene Capture by Cytochrome P450 3A4
  Full Text via DOI: 10.1124/mol.116.103721 Web of Science: 000378087600005
- 2016
  
  Photoswitchable anticancer activity via trans-cis isomerization of a combretastatin A-4 analog. ORGANIC & BIOMOLECULAR CHEMISTRY. 14:40-49.
  Full Text via DOI: 10.1039/c5ob02005k Web of Science: 000366861800004
- 2015
  
  Ab initio dynamics of the cytochrome P450 hydroxylation reaction. JOURNAL OF CHEMICAL PHYSICS. 142.
  Full Text via DOI: 10.1063/1.4907733 Web of Science: 000349847000027
- 2015
  
  Evidence for an Elevated Aspartate pK_a in the Active Site of Human Aromatase
  Full Text via DOI: 10.1074/jbc.M114.595108 Web of Science: 000347778200048
- 2014
  
  Spin equilibrium and O-2-binding kinetics of Mycobacterium tuberculosis CYP51 with mutations in the histidine-threonine dyad. JOURNAL OF INORGANIC BIOCHEMISTRY. 136:81-91.
  Full Text via DOI: 10.1016/j.jinorgbio.2014.03.017 Web of Science: 000337930100010
- 2013
  
  Cytochrome P450 compound I in the plane wave pseudopotential framework: GGA electronic and geometric structure of thiolate-ligated iron(IV)-oxo porphyrin. JOURNAL OF COMPUTATIONAL CHEMISTRY. 34:1647-1660.
  Full Text via DOI: 10.1002/jcc.23311 Web of Science: 000320389700004
- 2013
  
  Solvatochromism and the solvation structure of benzophenone. JOURNAL OF CHEMICAL PHYSICS. 138.
  Full Text via DOI: 10.1063/1.4809529 Web of Science: 000320675800025
- 2012
  
  A GGA plus U approach to effective electronic correlations in thiolate-ligated iron-oxo (IV) porphyrin. JOURNAL OF CHEMICAL PHYSICS. 137.
  Full Text via DOI: 10.1063/1.4755290 Web of Science: 000309428500019
- 2012
  
  Coupled Electron Transfer and Proton Hopping in the Final Step of CYP19-Catalyzed Androgen Aromatization. BIOCHEMISTRY. 51:3039-3049.
  Full Text via DOI: 10.1021/bi300017p Web of Science: 000302484200008
- 2011
  
  Tetrahydrofolate Recognition by the Mitochondrial Folate Transporter
  Full Text via DOI: 10.1074/jbc.M111.272187 Web of Science: 000294487500043
- 2010
  
  Free Energy Landscape of the Retinol/Serum Retinol Binding Protein Complex: A Biological Host-Guest System. JOURNAL OF PHYSICAL CHEMISTRY B. 114:11315-11322.
  Full Text via DOI: 10.1021/jp104103f Web of Science: 000281128700042
- 2010
  
  Acid/base and hydrogen bonding effects on the proton-coupled electron transfer of quinones and hydroquinones in acetonitrile: Mechanistic investigation by voltammetry, ¹H NMR and computation. ELECTROCHIMICA ACTA. 55:6507-6516.
  Full Text via DOI: 10.1016/j.electacta.2010.06.029 Web of Science: 000281501100025
- 2010
  
  Peroxo-Iron Mediated Deformylation in Sterol 14α-Demethylase Catalysis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 132:10293-10305.
  Full Text via DOI: 10.1021/ja906192b Web of Science: 000280456100027
- 2009
  
  Molecular Oxygen Activation and Proton Transfer Mechanisms in Lanosterol 14α-Demethylase Catalysis. JOURNAL OF PHYSICAL CHEMISTRY B. 113:8170-8182.
  Full Text via DOI: 10.1021/jp902932p Web of Science: 000266679200030
- 2007
  
  Development of keratinocyte growth factor receptor tyrosine kinase inhibitors for the treatment of cancer. ANTICANCER RESEARCH. 27:3801-3806.
  Web of Science: 000251901700004
- 2007
  
  Early events in the photochemistry of 2-naphthyl azide from femtosecond UV/Vis Spectroscopy and quantum chemical calculations: Direct observation of a very short-lived singlet nitrene. JOURNAL OF ORGANIC CHEMISTRY. 72:7581-7586.
  Full Text via DOI: 10.1021/jo070865w Web of Science: 000249698200012
- 2007
  
  Oxidative dehalogenation of perhalogenated benzenes by cytochrome P450 Compound I. BIOCHEMISTRY. 46:5924-5940.
  Full Text via DOI: 10.1021/bi700365x Web of Science: 000246456200008
- 2007
  
  Competition between α-cleavage and energy transfer in α-azidoacetophenones. JOURNAL OF ORGANIC CHEMISTRY. 72:2757-2768.
  Full Text via DOI: 10.1021/jo062160k Web of Science: 000245510600007
- 2006
  
  Early events in the photochemistry of aryl azides from femtosecond UV/vis spectroscopy and quantum chemical calculations. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 128:13402-13411.
  Full Text via DOI: 10.1021/ja061520i Web of Science: 000241157600034
- 2005
  
  Lead optimization of 7-benzyloxy 2-(4 '-pyridylmethyl)thio isoflavone aromatase inhibitors. BIOORGANIC & MEDICINAL CHEMISTRY. 13:6571-6577.
  Full Text via DOI: 10.1016/j.bmc.2005.07.038 Web of Science: 000232959200029
- 2005
  
  The direct detection of an aryl azide excited state:: An ultrafast study of the photochemistry of para- and ortho-biphenyl azide. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 127:13764-13765.
  Full Text via DOI: 10.1021/ja054135m Web of Science: 000232413300011
- 2005
  
  Synthesis and characterization of azole isoflavone inhibitors of aromatase. BIOORGANIC & MEDICINAL CHEMISTRY. 13:4063-4070.
  Full Text via DOI: 10.1016/j.bmc.2005.03.050 Web of Science: 000229666700019
- 2005
  
  The final catalytic step of cytochrome P450 aromatase: A density functional theory study. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 127:5224-5237.
  Full Text via DOI: 10.1021/ja044716w Web of Science: 000228229600049
- 2004
  
  Synthesis and aromatase inhibitory activity of novel pyridine-containing isoflavones. JOURNAL OF MEDICINAL CHEMISTRY. 47:4032-4040.
  Full Text via DOI: 10.1021/jm0306024 Web of Science: 000222856800016
Book Chapter
- 2017
  
  Molecular determinants of TRPC6 channel recognition by FKBP12. 69-96.
  Full Text via DOI: 10.1201/9781315207544-3
- 2017
  
  Molecular determinants of TRPC6 channel recognition by FKBP12. 69-96.
  Full Text via DOI: 10.1201/9781315207544
Conference
- 2014
  
  Molecular dynamics simulations of polytetrafluoroethylene at glassy transition temperature. 383-386.
- 2010
  
  Abstract P6-15-08: KGFR Tyrosine Kinase Inhibitors for the Treatment of Breast Cancer. CANCER RESEARCH. p6-15-08-p6-15-08.
  Full Text via DOI: 10.1158/0008-5472.sabcs10-p6-15-08
- 2010
  
  Free radical scavengers of natural and synthetic products. 67-74.
  Full Text via DOI: 10.17660/ActaHortic.2010.854.7
Other Scholarly Work
- 2023
  
  22nd International Conference on Cytochrome P450 (ICCP450). JOURNAL OF INORGANIC BIOCHEMISTRY.
  Full Text via DOI: 10.1016/j.jinorgbio.2023.112347 Web of Science: 001066169300001
- 2020
  
  PSI relieves the pressure of membrane fusion. JOURNAL OF BIOLOGICAL CHEMISTRY. 14563-14564.
  Full Text via DOI: 10.1074/jbc.H120.016038 Web of Science: 000588412800004
Review
- 2005
  
  Aromatase inhibitors in the treatment of breast cancer. ENDOCRINE REVIEWS. 331-345.
  Full Text via DOI: 10.1210/er.2004-0015 Web of Science: 000229127300005

principal investigator on

Dynamics and Interactions of Cytochrome P450 19A1 awarded by General Medical Sciences 2021 - 2025
Functional Dynamics of Cytochrome P4503A4 awarded by University of Washington 2021 - 2023

full name

John Hackett

ORCID iD

https://orcid.org/0000-0002-4923-2668 (confirmed)

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Co-author Network

Co-investigator Network