Selective ligands for the μ, δ, and κ opioid receptors identified from a single mixture based tetrapeptide positional scanning combinatorial library Article

Dooley, CT, Ny, P, Bidlack, JM et al. (1998). Selective ligands for the μ, δ, and κ opioid receptors identified from a single mixture based tetrapeptide positional scanning combinatorial library . JOURNAL OF BIOLOGICAL CHEMISTRY, 273(30), 18848-18856. 10.1074/jbc.273.30.18848

cited authors

  • Dooley, CT; Ny, P; Bidlack, JM; Houghten, RA

authors

abstract

  • A combinatorial library of 6,250,000 tetrapeptides in the mixture based positional scanning format was screened in binding assays for the three opioid receptors, μ, δ, and κ. Three different binding profiles were found. Individual peptides were synthesized representing all possible combinations of the active amino acids identified from the screening data. New, highly active peptides selective for each of the three receptors were chosen. This study demonstrates the power of mixture-based combinatorial libraries to identify distinctly different ligands for closely related receptors.

publication date

  • July 24, 1998

published in

Digital Object Identifier (DOI)

start page

  • 18848

end page

  • 18856

volume

  • 273

issue

  • 30