Identification of B cell and T cell epitopes using synthetic peptide combinatorial libraries Article

Pinilla, C, Appel, JR, Judkowski, V et al. (2012). Identification of B cell and T cell epitopes using synthetic peptide combinatorial libraries .(SUPPL.99), 10.1002/0471142735.im0905s99

cited authors

  • Pinilla, C; Appel, JR; Judkowski, V; Houghten, RA

authors

abstract

  • This unit presents a combinatorial library method that consists of the synthesis and screening of mixture-based synthetic combinatorial libraries of peptide molecules. The protocols employ peptide libraries to identify peptides recognized by MAbs and T cells. The first protocol uses a positional scanning peptide library made up of hexapeptides to identify antigenic determinants recognized by MAbs. The 120 mixtures in the hexapeptide library are tested for their inhibitory activity in a competitive ELISA. The second protocol uses a decapeptide library to identify T cell peptide ligands. The 200 mixtures of the decapeptide library are tested for their ability to induce T cell activation. Support protocols cover optimization of the assay conditions for each MAb or T cell, to achieve the best level of sensitivity and reproducibility, and preparation of a hexapeptide library, along with deconvolution approaches. © 2012 by John Wiley and Sons, Inc.

publication date

  • December 1, 2012

Digital Object Identifier (DOI)

issue

  • SUPPL.99