The use of synthetic peptide combinatorial libraries for the determination of peptide ligands in radio-receptor assays: Opioid peptides Article

Houghten, RA, Dooley, CT. (1993). The use of synthetic peptide combinatorial libraries for the determination of peptide ligands in radio-receptor assays: Opioid peptides . Bioorganic and Medicinal Chemistry Letters, 3(3), 405-412. 10.1016/S0960-894X(01)80222-5

cited authors

  • Houghten, RA; Dooley, CT

abstract

  • A synthetic peptide combinatorial library (SPCL) was prepared, composed of 52,128,400 L-amino acid hexapeptides, which was used with an iterative selection process to determine peptides capable of inhibiting binding of [3H] [D-Ala2,MePhe4,Gly-ol5]enkephalin (DAGO) to crude rat brain homogenates. The first five residues corresponded exactly to the naturally occurring opioid peptide sequences of methionine and leucine enkephalin. © 1993.

authors

publication date

  • January 1, 1993

published in

Digital Object Identifier (DOI)

start page

  • 405

end page

  • 412

volume

  • 3

issue

  • 3