Identification of related peptides recognized by a monoclonal antibody using a synthetic peptide combinatorial library Article

Appel, JR, Pinilla, C, Houghten, RA. (1992). Identification of related peptides recognized by a monoclonal antibody using a synthetic peptide combinatorial library . 1(1), 17-23. 10.1016/S1058-6687(05)80024-5

cited authors

  • Appel, JR; Pinilla, C; Houghten, RA

authors

abstract

  • Synthetic peptide combinatorial libraries (SPCLs) are composed of tens of millions of peptide sequences from which specific sequences can be systematically determined. The broad versatility of SPCLs residues in the fact that nonsupport-bound peptides are generated in quantities sufficient for use in the majority of existing bioassays. In the present study, an SPCL composed of more than 52 million peptides was screened with an antipeptide monoclonal antibody for the identification of peptide sequences that inhibit the interaction of the monoclonal antibody with its known antigenic peptide. Upon the completion of an iterative screening and selection process utilized in the SPCL approach, a number of peptide sequences that were related to the known antigenic determinant region were found. © 1992 Academic Press, Inc. All rights reserved.

publication date

  • January 1, 1992

Digital Object Identifier (DOI)

start page

  • 17

end page

  • 23

volume

  • 1

issue

  • 1