Activation of bee venom phospholipase A2 through a peptide-enzyme complex Article

Mingarro, I, Pérez-Payá, E, Pinilla, C et al. (1995). Activation of bee venom phospholipase A2 through a peptide-enzyme complex . FEBS LETTERS, 372(1), 131-134. 10.1016/0014-5793(95)00964-B

cited authors

  • Mingarro, I; Pérez-Payá, E; Pinilla, C; Appel, JR; Houghten, RA; Blondelle, SE

authors

abstract

  • Phospholipase A2 activation by membrane-bound peptides was investigated in order to understand the role of the membrane-induced conformation on activation, and to examine the occurrence of a peptide-enzyme complex at the lipid/water interface. For the peptides studies, bee venom phospholipase A2 was stimulated regardless of the membrane-bound conformation (α-helix, β-sheet or random coil). Using antisera raised against melittin, we were able to demonstrate the occurrence of a calcium-dependent complex involving the enzyme, phospholipid substrate, and peptide. © 1995.

publication date

  • September 18, 1995

published in

Digital Object Identifier (DOI)

start page

  • 131

end page

  • 134

volume

  • 372

issue

  • 1