VGCC responsiveness of SH-SY5Y human neuroblastoma cells in the PLLA three-dimensional microwell patterns
Article
An, Z, Huang, Q, Wang, Z et al. (2012). VGCC responsiveness of SH-SY5Y human neuroblastoma cells in the PLLA three-dimensional microwell patterns
. 25(3), 306-312. 10.3969/j.issn.1004-1699.2012.03.005
An, Z, Huang, Q, Wang, Z et al. (2012). VGCC responsiveness of SH-SY5Y human neuroblastoma cells in the PLLA three-dimensional microwell patterns
. 25(3), 306-312. 10.3969/j.issn.1004-1699.2012.03.005
UV lithography, silicon etching and soft lithography were adopted to fabricate three-dimensional poly-L-lactide (PLLA) microwell patterns. Human neuroblastoma (SH-SY5Y) cells were integrated with the PLLA patterns, and then cell growth behaviors and distribution were evaluated. Voltage-gated calcium channels(VGCCs) response of SH-SY5Y cells cultured in microwells and on flat PLLA substrates was detected by confocal microscopy with a calcium fluorescent indicator (Calcium Green-1, AM). It was found that SH-SY5Y cells distributed on patterns's top surface showed two-dimensional (2-D) adhesion behavior, while those cells attached to the microwell's side-wall and bottom surface showed three-dimensional (3-D) and boundary two-dimensional (B2-D) adhesion behaviors, respectively. In response to 50 mmol/L high K + depolarization, the percentage of 2-D cells on PLLA flat substrates with positive VGCCs response was 91. 2%, while this percentage was 75% and 81%, for 3-D and B2-D cells, respectively, significantly lower than that for 2-D cells. These results suggested that cell adhesion behaviors might be critical factors in affecting VGCCs responsiveness of SH-SY5 Y cells. Microwell patterns could be an effective way for engineering 3-D microenvironment for neural cell culture and establishing 3-D cell-based biosensing platforms.
