SJ 200, a Polyherbal formulation contains active constituents of various plants which were proved individually in the earlier research work effective for their antispasmodic activity. So in the present study we have evaluated SJ-200 for antispasmodic activity in gastrointestinal spasm on various smooth muscles in vitro and intestinal transit rate in vivo. SJ-200 inhibited spontaneous contraction of rabbit jejunum and also acetyl choline and barium chloride induced contraction dose dependently, 5-HT and acetyl choline induced contraction of rat fundus inhibited dose dependently. SJ-200 also inhibited acetyl choline and calcium chloride induced contraction or rat colon dose dependently. Oral administration of SJ-200 dose dependently reduced intestinal transit in mice when compared to atropine at 0.1mg/kg i.p. SJ-200 at (300 mg/kg) protected mice against diarrhoea induced by castor oil significantly as compared to control and standard loperamide at a dose of 5 mg/kg orally. In the present study it is concluded that SJ-200 inhibits the contraction produced by various spasmogens like acetylcholine, barium chloride, histamine, serotonin and calcium chloride. This suggests that the activity of SJ-200 is non-specific to any spasmogen.
