Development of a small animal pressure occluding device for mapping peripheral hemodynamic flow patterns in the murine tail
Conference
Dahal, A, Belilty Benmergui, S, Mijares, A et al. (2025). Development of a small animal pressure occluding device for mapping peripheral hemodynamic flow patterns in the murine tail
. 13306 10.1117/12.3042428
Dahal, A, Belilty Benmergui, S, Mijares, A et al. (2025). Development of a small animal pressure occluding device for mapping peripheral hemodynamic flow patterns in the murine tail
. 13306 10.1117/12.3042428
Vascular calcification (VC) is a major contributor to cardiovascular diseases, especially in patients with chronic kidney disease (CKD). Although various modalities exist for detecting vascular calcification, these imaging techniques remain expensive and not easily accessible. To address this issue, our lab developed a low-cost NIRS-based imaging technique (NIROS- near-infrared optical scanner) to detect VC by measuring the hemodynamic flow differences in the murine tail in response to occlusion. In our previous studies, a commercial occlusion cuff (CODA monitor) was used to occlude the murine tail and NIROS captured the hemodynamic changes in mice in response to total occlusion (250mmHg). However, this device lacked the ability to control the pressure and the occlusion paradigm. Herein, we custom-developed a small animal pressure occluding device capable of precise pressure control to allow venous, arterial, and total occlusion (including hypoxic conditions). The custom-pressure occlusion device can change the duration of occlusion, the maximum pressure and duration of occlusion, the duration of relaxation/deflation, and the duration between repeatable occlusion cycles. This allows induction of venous, arterial as well as total occlusions in a controllable manner. Our ongoing work is focused on determining the peripheral hemodynamic changes in response to various occlusion paradigms and determine the least pressure and distressing protocol that can help detect VC via studies on mouse models.
