The use of Positional Scanning Synthetic Peptide Combinatorial Libraries To Identify Immunological Relevant Peptides Book Chapter

Sospedra, M, Pinilla, C. (2006). The use of Positional Scanning Synthetic Peptide Combinatorial Libraries To Identify Immunological Relevant Peptides . 595-601. 10.1016/B978-012369442-3/50088-X

cited authors

  • Sospedra, M; Pinilla, C

authors

abstract

  • This chapter discusses the underlying principles of the use of positional scanning synthetic combinatorial peptide libraries. The systematic identification of ligands for biologically relevant receptors has been greatly facilitated by the introduction of combinatorial peptide chemistry. Many receptor-ligand interactions in biology involve the binding of peptidic molecules to complex surface receptors. Among these, the immune system is a particularly interesting example, since peptides are the main targets for T lymphocytes and can also be recognized by antibodies. Synthetic combinatorial libraries (SCLs) made up of mixtures having one position defined with a given amino acid (aa), while other positions have amino acids incorporated as mixtures. These libraries represent a very large number of compounds such as synthetic peptides. SCLs are generated by use of the multiple solid phase synthesis method known as the "tea bag approach." The assay data derived from positional library provides information about the most important aa for every diversity position of the Positional Scanning (PS)-SCL. © 2006 Elsevier Inc. All rights reserved.

publication date

  • December 1, 2006

Digital Object Identifier (DOI)

International Standard Book Number (ISBN) 13

start page

  • 595

end page

  • 601