Nondestructive Method for Determination of Water-Soluble Oxalate in Cultured Amaranthus tricolor Cells Article

Teutonico, RA, Knorr, D. (1985). Nondestructive Method for Determination of Water-Soluble Oxalate in Cultured Amaranthus tricolor Cells . JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 33(1), 60-62. 10.1021/jf00061a018

cited authors

  • Teutonico, RA; Knorr, D

authors

abstract

  • A nondestructive method was developed for screening cultured cells of Amaranthus tricolor to isolate low-oxalate variants, which upon regeneration could produce plants with improved bioavailability of calcium, iron, and zinc. Cells were chilled at 7 ± 1 °C for 45 min to enhance oxalate release, and methylated extracts were spotted on thin-layer chromatographic (TLC) plates that previously had been treated with a hydroxylamine hydrochloride—ferric chloride dye. Dimethyl oxalate spots were developed in an ammonia vapor and quantified with a densitometer. TLC determinations of the oxalate content of extracts gave reproducible results with a mean coefficient of variation of 4.51%. The TLC results correlated (P < 0.01) with oxalate data obtained from gas chromatographic analysis. In addition, cells were found to remain 77% viable after chilling. Thus, this screening method could be used effectively to isolate low-oxalate variants from cultured cells for the generation of low-oxalate A. tricolor cell lines. © 1985, American Chemical Society. All rights reserved.

publication date

  • January 1, 1985

Digital Object Identifier (DOI)

start page

  • 60

end page

  • 62

volume

  • 33

issue

  • 1