Targeted glycoproteomic identification of biomarkers for human breast carcinoma Article

Abbott, KL, Aoki, K, Lim, JM et al. (2008). Targeted glycoproteomic identification of biomarkers for human breast carcinoma . 7(4), 1470-1480. 10.1021/pr700792g

cited authors

  • Abbott, KL; Aoki, K; Lim, JM; Porterfield, M; Johnson, R; O'Regan, RM; Wells, L; Tiemeyer, M; Pierce, M

authors

abstract

  • Glycosylation is a dynamic post-translational modification that changes during the development and progression of various malignancies. During the oncogenesis of breast carcinoma, the glycosyltrans-ferase known as N-acetylglucosaminyltransferase Va (GnT-Va) transcript levels and activity are increased due to activated oncogenic signaling pathways. Elevated GnT-V levels leads to increased β(1,6)-branched N-linked glycan structures on glycoproteins that can be measured using a specific carbohydrate binding protein or lectin known as L-PHA. L-PHA does not bind to nondiseased breast epithelial cells, but during the progression to invasive carcinoma, cells show a progressive increase in L-PHA binding. We have developed a procedure for intact protein L-PHA-affinity enrichment, followed by nanospray ionization mass spectrometry (NSI-MS/MS), to identify potential biomarkers for breast carcinoma. We identified L-PHA reactive glycoproteins from matched normal (nondiseased) and malignant tissue isolated from patients with invasive ductal breast carcinoma. Comparison analysis of the data identified 34 proteins that were enriched by L-PHA fractionation in tumor relative to normal tissue for at least 2 cases of ductal invasive breast carcinoma. Of these 34 L-PHA tumor enriched proteins, 12 are common to all 4 matched cases analyzed. These results indicate that lectin enrichment strategies targeting a particular glycan change associated with malignancy can be an effective method of identifying potential biomarkers for breast carcinoma. © 2008 American Chemical Society.

publication date

  • April 1, 2008

Digital Object Identifier (DOI)

start page

  • 1470

end page

  • 1480

volume

  • 7

issue

  • 4