N-acetylglucosaminyltranferase VB expression enhances β1 integrin- dependent PC12 neurite outgrowth on laminin and collagen Article

Lee, I, Guo, HB, Kamar, M et al. (2006). N-acetylglucosaminyltranferase VB expression enhances β1 integrin- dependent PC12 neurite outgrowth on laminin and collagen . JOURNAL OF NEUROCHEMISTRY, 97(4), 947-956. 10.1111/j.1471-4159.2006.03785.x

cited authors

  • Lee, I; Guo, HB; Kamar, M; Abbott, K; Troupe, K; Lee, JK; Alvarez-Manilla, G; Pierce, M

authors

abstract

  • N-acetylglucosaminyltransferase VB (GnT-VB, -IX) is a newly discovered glycosyltransferase expressed exclusively in high levels in neuronal tissue during early development. Its homolog, GnT-V, is expressed in many tissues and modulates cell-cell and cell-matrix adhesion. The ability of GnT-VB to regulate cell-matrix interactions was initially investigated using the rat pheochromocytoma PC12 neurite outgrowth model. PC12 cells stably transfected with GnT-VB consistently showed an enhanced rate of nerve growth factor (NGF)-induced neurite outgrowth on collagen and laminin substrates. Levels of TrkA receptor phosphorylation and downstream ERK activation induced by NGF were not influenced by GnT-VB expression. No significant difference was observed in the rate of neurite outgrowth when cells were cultured on non-coated culture dishes, indicating that integrin-ECM interaction is required for the stimulatory effects. Neurite outgrowth induced by manganese-dependent activation of β1 integrin on collagen and laminin substrates, however, showed a significant increase in neurite length for the PC12/GnT-VB cells, compared with control cells, suggesting that the enhancement is most likely mediated by alteration of β1 integrin-ECM interaction by GnT-VB. These results demonstrate that GnT-VB expression can modulate the rate of neurite outgrowth by affecting β1 integrin-ECM interaction. © 2006 International Society for Neurochemistry.

publication date

  • May 1, 2006

published in

Digital Object Identifier (DOI)

start page

  • 947

end page

  • 956

volume

  • 97

issue

  • 4