HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro
Article
Green, DF, Resnick, L, Bourgoignie, JJ. (1992). HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro
. KIDNEY INTERNATIONAL, 41(4), 956-960. 10.1038/ki.1992.146
Green, DF, Resnick, L, Bourgoignie, JJ. (1992). HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro
. KIDNEY INTERNATIONAL, 41(4), 956-960. 10.1038/ki.1992.146
The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.
