Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening Article

Kim, KM, Adyshev, DM, Kása, A et al. (2013). Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening . MICROVASCULAR RESEARCH, 88 19-24. 10.1016/j.mvr.2013.04.002

cited authors

  • Kim, KM; Adyshev, DM; Kása, A; Zemskov, EA; Kolosova, IA; Csortos, C; Verin, AD

authors

abstract

  • We have previously demonstrated that PKC-potentiated inhibitory protein of protein phosphatase-1 (CPI-17) is expressed in lung endothelium. CPI-17, a specific inhibitor of myosin light chain phosphatase (MLCP), is involved in the endothelial cytoskeletal and barrier regulation. In this paper, we report the identification of fourteen putative CPI-17 interacting proteins in the lung using BacterioMatch Two-Hybrid System. Five of them: plectin 1 isoform 1, alpha II spectrin, OK/SW-CL.16, gelsolin isoform a, and junction plakoglobin are involved in actin cytoskeleton organization and cell adhesion, suggesting possible significance of these binding partners in CPI-17-mediated cytoskeletal reorganization of endothelial cells. Furthermore, we confirmed the specific interaction between plakoglobin and CPI-17, which is affected by the phosphorylation status of CPI-17 in human lung microvascular endothelial cells. © 2013 Elsevier Inc.

publication date

  • July 1, 2013

published in

Digital Object Identifier (DOI)

start page

  • 19

end page

  • 24

volume

  • 88