Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate
Article
Kao, HI, Henricksen, LA, Liu, Y et al. (2002). Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate
. JOURNAL OF BIOLOGICAL CHEMISTRY, 277(17), 14379-14389. 10.1074/jbc.M110662200
Kao, HI, Henricksen, LA, Liu, Y et al. (2002). Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate
. JOURNAL OF BIOLOGICAL CHEMISTRY, 277(17), 14379-14389. 10.1074/jbc.M110662200
Flap endonuclease 1 (FEN1) is a structure-specific nuclease that cleaves substrates containing unannealed 5′-flaps during Okazaki fragment processing. Cleavage removes the flap at or near the point of annealing. The preferred substrate for archaeal FEN1 or the 5′-nuclease domains of bacterial DNA polymerases is a double-flap structure containing a 3′-tail on the upstream primer adjacent to the 5′-flap. We report that FEN1 in Saccharomyces cerevisiae (Rad27p) exhibits a similar specificity. Cleavage was most efficient when the upstream primer contained a 1-nucleotide 3′-tail as compared with the fully annealed upstream primer traditionally tested. The site of cleavage was exclusively at a position one nucleotide into the annealed region, allowing human DNA ligase I to seal all resulting nicks. In contrast, a portion of the products from traditional flap substrates is not ligated. The 3′-OH of the upstream primer is not critical for double-flap recognition, because Rad27p is tolerant of modifications. However, the positioning of the 3′-nucleotide defines the site of cleavage. We have tested substrates having complementary tails that equilibrate to many structures by branch migration. FEN1 only cleaved those containing a 1-nucleotide 3′-tail. Equilibrating substrates containing 12-ribonucleotides at the end of the 5′-flap simulates the situation in vivo. Rad27p cleaves this substrate in the expected 1-nucleotide 3′-tail configuration. Overall, these results suggest that the double-flap substrate is formed and cleaved during eukaryotic DNA replication in vivo.
