Enzyme Linked Immunosorbent Assay (ELISA) for β-endorphin and its antibodies Article

Sarma, JK, Hoffmann, SR, Houghten, RA. (1986). Enzyme Linked Immunosorbent Assay (ELISA) for β-endorphin and its antibodies . LIFE SCIENCES, 38(19), 1723-1732. 10.1016/0024-3205(86)90122-0

cited authors

  • Sarma, JK; Hoffmann, SR; Houghten, RA

authors

abstract

  • An Enzyme Linked Immunosorbent Assay (ELISA) is described for use in the determination of β-endorphin antibody titers, as well as for the quantitation of naturally occuring levels of β-endorphin in plasma and other bodily fluids. The ability of the assay to accomodate unpurified samples containing small concentrations of β-endorphin was improved through the use of affinity purified antibodies in conjunction with a competitive inhibition ELISA. The problem of non-specific binding of β-endorphin during competitive inhibition assays was circumvented through a two-step process in which the plate was first coated with BSA, followed by a second plate coating with poly-lysine (MW4000). The second coating with poly-lysine was found necessary in order to eliminate intermolecular void spaces following initial plate treatment with BSA. Following these procedures enabled quantitation of β-EP at a level as low as 10pmoles per microtitre plate well. © 1986.

publication date

  • May 12, 1986

published in

Digital Object Identifier (DOI)

start page

  • 1723

end page

  • 1732

volume

  • 38

issue

  • 19