Human somatotropin. Reaction with hydrogen peroxide Article

Houghten, RA, Glaser, CB, Li, CH. (1977). Human somatotropin. Reaction with hydrogen peroxide . ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 178(2), 350-355. 10.1016/0003-9861(77)90203-X

cited authors

  • Houghten, RA; Glaser, CB; Li, CH

authors

abstract

  • Two methionine-modified derivatives of human somatotropin have been prepared by oxidation of the methionines with H2O2 to sulfoxide. The monomeric derivatives were characterized by exclusion chromatography, amino acid composition, circular dichroism spectra, relative rates of tryptic digestion, and biological property. Partially oxidized somatotropin, with two of its three methionines oxidized, was found to be similar to the native hormone by all criteria examined except that the growth promoting potency was reduced to 25% of the native hormone. The unoxidized methionine in this derivative was found to be the residue at position 170. The derivative in which all of the methionines had been oxidized showed major changes in all physical parameters examined as well as significant loss of the growth-promoting activity. © 1977.

publication date

  • January 30, 1977

published in

Digital Object Identifier (DOI)

start page

  • 350

end page

  • 355

volume

  • 178

issue

  • 2