ISOLATION OF α‐MELANOTROPIN AND N, O‐DIACETYLSERINE1‐α‐MELANOTROPIN FROM PORCINE PITUITARY EXTRACTS Article

BUCKLEY, DI, HOUGHTEN, RA, RAMACHANDRAN, J. (1981). ISOLATION OF α‐MELANOTROPIN AND N, O‐DIACETYLSERINE1‐α‐MELANOTROPIN FROM PORCINE PITUITARY EXTRACTS . 17(4), 508-513. 10.1111/j.1399-3011.1981.tb02021.x

cited authors

  • BUCKLEY, DI; HOUGHTEN, RA; RAMACHANDRAN, J

authors

abstract

  • In the course of isolation of α‐melanotropin (α‐MSH) from porcine pituitary extracts, a peptide with identical amino acid composition but slightly less polar properties compared to α‐MSH was detected and isolated by partition chromatography. The new peptide was identified as N, O‐diacetyl‐Ser1 ‐α‐MSH by high resolution nuclear magnetic resonance spectroscopy. N, O‐diacetyl‐Ser1 ‐α‐MSH was readily converted to α‐MSH by incubation at pH 9.5. The O‐acetyl analog of α‐MSH was found to be as active as α‐MSH in stimulating lipolysis in isolated rabbit adipocytes. © 1981 Munksgaard, Copenhagen

publication date

  • January 1, 1981

Digital Object Identifier (DOI)

start page

  • 508

end page

  • 513

volume

  • 17

issue

  • 4