Preparative isotachophoresis on polyacrylamide gels: Resolution as a function of time and wattage Article

Houghten, R, Chrambach, A. (1977). Preparative isotachophoresis on polyacrylamide gels: Resolution as a function of time and wattage . ANALYTICAL BIOCHEMISTRY, 77(1), 303-309. 10.1016/0003-2697(77)90319-0

cited authors

  • Houghten, R; Chrambach, A

abstract

  • The effect of migration path length and wattage on resolution in isotachophoresis on polyacrylamide gels of the model proteins, hemoglobin and bovine serum albumin, was investigated. Resolution appears optimal as soon as the steady state is reached, irrespective of whether the steady state is attained rapidly at high wattages and short migration path or more slowly at low wattages and a longer migration path. The steady state of isotachophoresis, like that of isoelectric focusing, appears to decay in proportion to time and wattage. A milligram-preparative prototype apparatus suitable for elution of zones separated by isotachophoresis on polyacrylamide gel was developed and tested. © 1977.

publication date

  • January 1, 1977

published in

Digital Object Identifier (DOI)

start page

  • 303

end page

  • 309

volume

  • 77

issue

  • 1