Soluble Combinatorial Libraries: Extending the Range and Repertoire of Chemical Diversity Article

Houghten, RA. (1994). Soluble Combinatorial Libraries: Extending the Range and Repertoire of Chemical Diversity . 6(4), 354-360. 10.1006/meth.1994.1035

cited authors

  • Houghten, RA

authors

abstract

  • The generation and use of libraries made up of millions of chemical entities and the ability to identify the active compound in such libraries are at the forefront of a revolution in drug discovery and basic research. Such libraries, when made up of peptide sequences, offer a fundamental, practical advance in the study of interactions between peptides and their biochemical or pharmacological targets. The utility of soluble peptide libraries ranging from three to eight amino acids in length, and made up of mixtures from 361 tripeptides to 200 billion decapeptides, is described. These are readily usable in virtually all in vitro (and even in vivo) assay systems. The examples presented illustrate the utility of soluble peptide libraries for the study of antibody/antigen interactions, the identification of highly active opioid peptides in receptor binding studies using crude rat brain homogenates, and in vivo studies, in which the peptide mixtures making up the library are administered intravenously to determine peptide sequences that affect heart rate and blood pressure. A new class of library is also described, termed a modified peptide library, which is used to determine potent anti-Staphylococcus aureus compounds. © 1994 Academic Press, Inc.

publication date

  • January 1, 1994

Digital Object Identifier (DOI)

start page

  • 354

end page

  • 360

volume

  • 6

issue

  • 4