Antibodies of predetermined specificity against chemically synthesized peptides of human interleukin 2
Article
Altman, A, Cardenas, JM, Houghten, RA et al. (1984). Antibodies of predetermined specificity against chemically synthesized peptides of human interleukin 2
. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 81(7 I), 2176-2180. 10.1073/pnas.81.7.2176
Altman, A, Cardenas, JM, Houghten, RA et al. (1984). Antibodies of predetermined specificity against chemically synthesized peptides of human interleukin 2
. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 81(7 I), 2176-2180. 10.1073/pnas.81.7.2176
This communication describes the preparation of antibodies to human interleukin 2 (IL-2), using as immunogens synthetic peptides derived from the predicted amino acid sequences of IL-2. Rabbits and mice were immunized with protein carrier conjugates of eight chemically synthesized IL-2-derived peptides, each consisting of 13-15 amino acids. The immune antisera were screened in a solid-phase ELISA for reactivity to a native human IL-2. Antibodies to four of the eight peptides were found by a variety of biological and immunochemical criteria to react against human IL-2. Furthermore, an affinity-purified antibody to one of the IL-2 peptides (peptide 84) specifically stained the cytoplasm of phytohemagglutinin-stimulated human peripheral blood lymphocytes or T-leukemic cells (Jurkat). Antibodies to synthetic IL-2 peptides should serve as useful probes for studying this lymphokine and for developing quantitative assays for measuring its levels in biological fluids and its association with disease.
