The use of synthetic peptides in the formation of biophysically and biologically active pulmonary surfactants Article

Revak, SD, Merritt, TA, Hallman, M et al. (1991). The use of synthetic peptides in the formation of biophysically and biologically active pulmonary surfactants . PEDIATRIC RESEARCH, 29(5), 460-465. 10.1203/00006450-199105010-00010

cited authors

  • Revak, SD; Merritt, TA; Hallman, M; Heldt, G; La Polla, RJ; Hoey, K; Houghten, RA; Cochrane, CG

authors

abstract

  • Synthetic pulmonary surfactants consisting of mixtures of phospholipids with synthetic peptides based on the amino acid sequence of human surfactant apoprotein SP-B were prepared. These surfactants were analyzed for their ability to lower surface tension on a pulsating bubble surfactometer and for their capacity to improve lung compliance and increase alveolar expansion in a fetal rabbit model of surfactant deficiency. The data demonstrate that several peptides, ranging from 17 to 45 residues in length, matching the carboxy-terminal sequence of the SP-B protein, when appropriately recombined with the phospholipids dipalmitoylphosphatidylcholine and phosphatidyl-glycerol (3:1), are capable of producing a synthetic surfactant with biophysical and biologic activity approaching that of human surfactant derived from amniotic fluid. © 1991 International Pediatric Research Foundation, Inc.

publication date

  • January 1, 1991

published in

Digital Object Identifier (DOI)

start page

  • 460

end page

  • 465

volume

  • 29

issue

  • 5