The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands Article

Dooley, CT, Houghten, RA. (1993). The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands . LIFE SCIENCES, 52(18), 1509-1517. 10.1016/0024-3205(93)90113-H

cited authors

  • Dooley, CT; Houghten, RA

authors

abstract

  • The application of a new synthetic peptide combinatorial library (SPCL) is described. This library, termed a positional scanning SPCL, contains six positional SPCLs, each of which contains all possible hexameric combinations of 18 of the 20 natural L-amino acids (186 = 34, 012, 224 peptides). Each positional SPCL (O1XXXXX-NH2, XO2XXXX-NH2, XXO3XXX-NH2, XXXO4XX-NH2, XXXXO5X-NH2, and XXXXXO6-NH2) was used to determine the most active amino acid for the six positions of a hexamer. Combinations of these amino acids were used to synthesize 24 individual peptides, which were then tested for activity. The most active peptide found corresponded to a hexameric analogue of methionine-enkephalin. Results obtained in this study are compared to those obtained using the SPCL described earlier (1) (O1O2XXXX-NH2), and the subsequent iterative process. © 1993.

publication date

  • January 1, 1993

published in

Digital Object Identifier (DOI)

start page

  • 1509

end page

  • 1517

volume

  • 52

issue

  • 18