Acetalins: Opioid receptor antagonists determined through the use of synthetic peptide combinatorial libraries
Article
Dooley, CT, Chung, NN, Schiller, PW et al. (1993). Acetalins: Opioid receptor antagonists determined through the use of synthetic peptide combinatorial libraries
. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 90(22), 10811-10815. 10.1073/pnas.90.22.10811
Dooley, CT, Chung, NN, Schiller, PW et al. (1993). Acetalins: Opioid receptor antagonists determined through the use of synthetic peptide combinatorial libraries
. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 90(22), 10811-10815. 10.1073/pnas.90.22.10811
A synthetic peptide combinatorial library made up of 52,128,400 hexapeptides, each having an acetyl group at the N terminus and an amide group on the C terminus, was screened to find compounds able to displace tritiated [D-Ala2,MePhe4,Gly-ol5]enkephalin from μ opioid receptor binding sites in crude rat brain homogenates. Individual peptides with μ receptor affinity were found using an iterative process for successively determining the most active peptide mixtures. Upon completion of this iterative process, the three peptides with the highest affinity were Ac-RFMWMT-NH2, Ac-RFMWMR-NH2, and Ac-RFMWMK-NH2. These peptides showed high affinity for μ and κ3 opioid receptors, somewhat lower affinity for δ receptors, weak affinity for κ1 receptors, and no affinity for κ2 receptors. They were found to be potent μ receptor antagonists in the guinea pig ileum assay and relatively weak antagonists in the mouse vas deferens assay. These peptides represent a class of opioid receptor ligands that we have termed acetalins (acetyl plus enkephalin).
