Induction of murine cytotoxic T lymphocytes against Plasmodium falciparum sporozoite surface protein 2 Article

Wizel, B, Rogers, WO, Houghten, RA et al. (1994). Induction of murine cytotoxic T lymphocytes against Plasmodium falciparum sporozoite surface protein 2 . 24(7), 1487-1495. 10.1002/eji.1830240705

cited authors

  • Wizel, B; Rogers, WO; Houghten, RA; Lanar, DE; Tine, JA; Hoffman, SL

authors

abstract

  • Sporozoite surface protein 2 has been identified as a target of malaria vaccines designed to produce protective CD8+ cytotoxic T lymphocytes (CTL) because mice immunized with mastocytoma cells expressing a fragment of Plasmodium yoelii sporozoite surface protein 2 (PySSP2) are protected against malaria by an immune response that requires CD8+ CTL. To define CTL epitopes in the Plasmodium falciparum sporozoite surface protein 2 (PfSSP2), spleen cells (SC) from mice immunized with irradiated sporozoites (irr spz), were stimulated with synthetic peptides, and these effectors were tested for cytolytic activity against peptide‐pulsed, major histocompatibility complex (MHC)‐matched targets. Two peptides containing CTL epitopes, A6 (Pf SSP2 3D7 214–233) and BH1 (Pf SSP2 3D7 3–11) were identified in bulk cultures of SC from immune C57BL/6 mice, and by production of CTL lines. Immunization with recombinant vaccinia expressing the full length PfSSP2 induced antigen specific, MHC‐restricted, CD8+ T cell‐dependent cytolytic activity against these two peptides. Finally, CTL were induced by immunization with a bacteria‐derived recombinant fragment of PfSSP2 (rPfSSP2) mixed with a liposomal formulation containing a cationic lipid (Lipofectin® Reagent, LPF). Induced CTL lysed target cells pulsed with peptide A6 or with LPF/rPfSSP2, but not targets pulsed with only rPfSSP2. These studies demonstrate that CTL specific to PfSSP2 are present in C57BL/6 mice and that immunization with purified rPfSSP2 delivered with LPF induces a cytotoxic T cell response. Copyright © 1994 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

publication date

  • January 1, 1994

Digital Object Identifier (DOI)

start page

  • 1487

end page

  • 1495

volume

  • 24

issue

  • 7