T cell multideterminant regions in the human immunodeficiency virus envelope: Toward overcoming the problem of major histocompatibility complex restriction Article

Hale, PM, Cease, KB, Houghten, RA et al. (1989). T cell multideterminant regions in the human immunodeficiency virus envelope: Toward overcoming the problem of major histocompatibility complex restriction . 1(4), 409-415. 10.1093/intimm/1.4.409

cited authors

  • Hale, PM; Cease, KB; Houghten, RA; Ouyang, C; Putney, S; Javaherian, K; Margalit, H; Cornette, JL; Spouge, JL; Delisi, C; Berzofsky1, JA

abstract

  • Helper T cell determinants should be an Important component of an anti-human Immunodeficiency virus (HIV) vaccine aimed at either antibody or cytotoxic T cell Immunity. However, model protein studies have raised concern about the usefulness of any single determinant, because a given determinant is likely to be seen by only a small subset of major histocompatibility complex (MHC) types within the population. Here, we use 44 peptides, including ones predicted and not predicted on the basis of amphipathicity to be potential T cell sites, to locate T cell antigenic determinants recognized by mice of four MHC haplotypes immunized with the whole gp160 envelope protein. Although the preselection of peptides necessitates caution In a statistical analysis, α-amphipathic peptides predominated among sites eliciting the strongest response. Although we have not tested the entire sequence, we have identified six multldetermlnant regions, in which overlapping peptides are recognized by mice of either three or all four MHC types. Four of the six regions have sequences relatively conserved among HIV-1 isolates. The existence of such multideterminant regions recognized by multiple MHC haplotypes suggests the possibility that use of peptides longer than a minimal determinant and containing several overlapping determinants may be a possible approach to circumvent the serious problem of MHC restriction in peptide vaccines aimed at eliciting T cell immunity. © 1989 The Japanese Society for Immunology.

publication date

  • January 1, 1989

Digital Object Identifier (DOI)

start page

  • 409

end page

  • 415

volume

  • 1

issue

  • 4