Induction of broadly cross-reactive cytotoxic T cells recognizing an HIV-1 envelope determinant Article

Takahashi, H, Nakagawa, Y, Pendleton, CD et al. (1992). Induction of broadly cross-reactive cytotoxic T cells recognizing an HIV-1 envelope determinant . SCIENCE, 255(5042), 333-336.

cited authors

  • Takahashi, H; Nakagawa, Y; Pendleton, CD; Houghten, RA; Yokomuro, K; Germain, RN; Berzofsky, JA

abstract

  • An immunodominant determinant for cytotoxic T lymphocytes (CTLs) exists in the hypervariable portion of human immunodeficiency virus-1 (HIV-1) gp160. Three mouse CTL lines (specific for isolates MN, RF, and IIIB) were examined for recognition of homologous determinants from distinct isolates. Only MN-elicited CTLs showed extensive interisolate cross-reactivity. Residue 325 played a critical role in specificity, with MN-elicited CTLs responding to peptides with an aromatic or cyclic residue and IIIB-induced cells recognizing peptides with an aliphatic residue at this position. CTL populations with broad specificities were generated by restimulation of IIIB-gp160 primed cells with MN-type peptides that have an aliphatic substitution at 325. This represents an approach to synthetic vaccines that can generate broadly cross-reactive CTLs capable of effector function against a wide range of HIV isolates.

publication date

  • January 17, 1992

published in

start page

  • 333

end page

  • 336

volume

  • 255

issue

  • 5042