Detection of high molecular weight forms of platelet-derived growth factor by sequence-specific antisera Article

Niman, HL, Houghten, RA, Bowen-Pope, DF. (1984). Detection of high molecular weight forms of platelet-derived growth factor by sequence-specific antisera . SCIENCE, 226(4675), 701-703. 10.1126/science.6494905

cited authors

  • Niman, HL; Houghten, RA; Bowen-Pope, DF

abstract

  • Antisera to synthetic peptides representing sequences of both chains of platelet-derived growth factor (PDGF) were used to structurally analyze PDGF isolated from outdated human platelets and PDGF-like proteins in normal and transformed cells. Most PDGF isolated from platelets did not contain the carboxyl portion of PDGF-2 in contrast to p20sis, the major form of p28sis detected in simian sarcoma virus-transformed cells. In addition, higher molecular weight forms of molecules containing PDGF-I and PDGF-2 sequences were detected in all cell lines tested. These lines were heterogeneous with respect to species, cell type, and transforming agent.

publication date

  • January 1, 1984

published in

Digital Object Identifier (DOI)

start page

  • 701

end page

  • 703

volume

  • 226

issue

  • 4675