Epitopes of the Mycobacterium tuberculosis 65-kilodalton protein antigen as recognized by human T cells
Article
Oftung, F, Mustafa, AS, Shinnick, TM et al. (1988). Epitopes of the Mycobacterium tuberculosis 65-kilodalton protein antigen as recognized by human T cells
. JOURNAL OF IMMUNOLOGY, 141(8), 2749-2754.
Oftung, F, Mustafa, AS, Shinnick, TM et al. (1988). Epitopes of the Mycobacterium tuberculosis 65-kilodalton protein antigen as recognized by human T cells
. JOURNAL OF IMMUNOLOGY, 141(8), 2749-2754.
A synthetic peptide approach has been used to identify the epitopes recognized by clonal and polyclonal human T cells reactive to the recombinant mycobacterial 65-kDa protein Ag. Three of the four epitopes identified were recognized as cross-reactive between Mycobacterium tuberculosis and Mycobacterium leprae, although their amino acid sequence in two of three cases was not identical. The peptide (231-245) defining an epitope recognized as specific to the M. tuberculosis complex contains two substitutions compared with the homologous M. leprae region of which one or both are critical to T cell recognition. The reactive T cell clones showed helper/inducer phenotype (CD4+, CD8-), and secrete IL-2, granulocyte-macrophage-CSf, and IFN-γ upon Ag stimulation. The same clones display cytotoxicity against macrophages pulsed with the relevant peptides or mycobacteria.
