Specificity of Rabbit Antibodies Elicited by Related Synthetic Peptides Article

Chersi, A, Zito, R, Houghten, RA et al. (1986). Specificity of Rabbit Antibodies Elicited by Related Synthetic Peptides . 41(5-6), 613-617. 10.1515/znc-1986-5-620

cited authors

  • Chersi, A; Zito, R; Houghten, RA; Chillemi, F; Centis, D

abstract

  • Three 17-residue peptides, presenting from 65% to 70% sequence homology, and one endecapeptide, with no apparent homology with the first three, were chemically synthesized and investigated in their ability to elicit rabbit antipeptide antibodies. The complex crossreactivities of the antisera were investigated by testing the binding of the antibodies to the intact peptides, to their enzymatic fragments, and by the use of specific immunoadsorbents. Antipeptide antibodies may or may not crossreact with related “parent” peptides, this depending upon number, distribution, and localization of amino acid differences in low or high antigenicity regions of the immunogen. Related peptides may elicit antibodies that crossreact almost completely, and therefore not specific for one or the other “parent” peptide. Those antibodies may therefore be of little use for the selective recognition of closely related structures. © 1986, Walter de Gruyter. All rights reserved.

publication date

  • June 1, 1986

Digital Object Identifier (DOI)

start page

  • 613

end page

  • 617

volume

  • 41

issue

  • 5-6