Mucosal antitoxin response in volunteers to immunization with a synthetic peptide of Escherichia coli heat-stable enterotoxin Article

Klipstein, FA, Engert, RF, Houghten, RA. (1985). Mucosal antitoxin response in volunteers to immunization with a synthetic peptide of Escherichia coli heat-stable enterotoxin . INFECTION AND IMMUNITY, 50(1), 328-332. 10.1128/iai.50.1.328-332.1985

cited authors

  • Klipstein, FA; Engert, RF; Houghten, RA

authors

abstract

  • Peroral immunization of volunteers on four weekly occasions with 750 μg of a conjugate containing 3,000 antigen units of a synthetically produced peptide of hyperantigenic Escherichia coli heat-stable (ST) toxin, conjugated with the heat-labile toxin B subunit as a carrier, raised serum immunoglobulin G antitoxin titers to ST by fourfold and intestinal immunoglobulin A antitoxin titers to ST by sevenfold over control values at five weeks postimmunization. The ability of jejunal aspirates from the immunized volunteers to neutralize ST in the suckling mouse assay correlated with the intestinal immunoglobulin A ST antitoxin response determined by enzyme-linked immunosorbent assay.

publication date

  • January 1, 1985

published in

Digital Object Identifier (DOI)

start page

  • 328

end page

  • 332

volume

  • 50

issue

  • 1