Noncoding small RNAs (sRNAs) are increasingly recognized as important regulators of cellular processes in both eukaryotes and prokaryotes. The human opportunistic pathogen Pseudomonas aeruginosa is one such example where sRNAs have been identified as critical elements of cellular adaptation and virulence. P. aeruginosa is known for its ubiquitous presence, remarkable battery of virulence factors, and ability to resist antibiotics. A complex network of transcriptional factors controls the physiology and virulence in P. aeruginosa. In the last two decades, many regulatory sRNAs have been identified at the core of these networks. In this chapter, we summarize the role of sRNAs in regulating the critical pathways that are vital for P. aeruginosa survival and pathogenesis. Key processes such as acute-chronic phenotype transition, iron uptake, catabolite repression, and stress responses in P. aeruginosa are discussed, highlighting the importance of sRNAs.