Intraoperative spectroscopic-guided brain tumor resection: Reduction of blood contamination effects on tissue optical spectra Conference

Lin, WC, Mahadevan-Jansen, A, Jansen, ED. (2001). Intraoperative spectroscopic-guided brain tumor resection: Reduction of blood contamination effects on tissue optical spectra . SMART BIOMEDICAL AND PHYSIOLOGICAL SENSOR TECHNOLOGY XI, 4254 181-186. 10.1117/12.427932

cited authors

  • Lin, WC; Mahadevan-Jansen, A; Jansen, ED

authors

abstract

  • An optical spectroscopy-based system for intraoperative guidance of brain tumor resection is currently under development at Vanderbilt University and Vanderbilt University Medical Center. The effectiveness of brain tumor margin detection, especially the infiltrating type, using combined in vivo autofluorescence and diffuse reflectance spectroscopy was successfully demonstrated in a preliminary clinical trial (on a total of 26 brain tumor patients). In this trial, a two-step empirical discrimination algorithm based on autofluorescence and diffuse reflectance intensity at 460 nm and 625 nm yielded 100% sensitivity and 76% specificity in differentiating infiltrating tumor margins from normal brain tissues. Moreover, optical spectroscopy was also found to be more sensitive in identifying infiltrating tumor margins in comparison with the existing technologies such as the frameless surgical navigation systems. Blood contamination was identified as one of the major obstacles in accurate in vivo brain tissue differentiation using optical spectroscopy. Hence a simple but effective technique capable of minimizing the effect of blood contamination on tissue optical spectra was developed. The theoretical basis of this technique was developed wherein the ratio of fluorescence intensity and the h-th power of diffuse reflectance intensity at a given emission wavelength, was found to be immune to spectral distortions induced by blood contamination, Here, h is determined by the absorption coefficients of whole blood at the excitation and emission wavelengths. The validity of this theory was confirmed using Monte Carlo simulations as well as in an experimental study.

publication date

  • January 1, 2001

Digital Object Identifier (DOI)

start page

  • 181

end page

  • 186

volume

  • 4254