The function of PP2A/B56 in non-metazoan multicellular development Article

Lee, NS, Veeranki, S, Kim, B et al. (2008). The function of PP2A/B56 in non-metazoan multicellular development . DIFFERENTIATION, 76(10), 1104-1110. 10.1111/j.1432-0436.2008.00301.x

cited authors

  • Lee, NS; Veeranki, S; Kim, B; Kim, L

authors

abstract

  • DB56, the Dictyostelium B56 homolog, displayed high sequence homology to other eukaryotic B56 subunits of the PP2A and a specific association with the PP2A catalytic subunit. Cells lacking DB56, psrA-, displayed higher PP2A phosphatase activity compared with the wild type, ∼10 hr of delayed expression of ecmA and ecmB prestalk markers, and inefficient culmination. The prespore marker cotB declined as wild-type cells culminate, but no such decline was observed from psrA- cells. Interestingly, psrA- cells exhibited higher GSK3 kinase activity. Furthermore, the expression of the dominant negative GSK3 mutant (K84/85M) in psrA- cells improved both prestalk and prespore expression patterns similarly to wild-type cells. However, culmination was not restored in psrA- cells expressing dominant negative GSK3, which suggests that PP2A/DB56 has an extra target during terminal differentiation. This report shows that PP2A/DB56 controls not only metazoan development, but also non-metazoan cell fate decision processes. © 2008, Copyright the Authors.

publication date

  • January 1, 2008

published in

Digital Object Identifier (DOI)

start page

  • 1104

end page

  • 1110

volume

  • 76

issue

  • 10