Peak height determination in analysis of chlortetracycline in animal feeds Article

Hudson, SE, Cohen, ME, Hudson, DL et al. (1987). Peak height determination in analysis of chlortetracycline in animal feeds . CHROMATOGRAPHIA, 24(1), 291-294. 10.1007/BF02688492

cited authors

  • Hudson, SE; Cohen, ME; Hudson, DL; Gump, B

authors

abstract

  • In an HPLC method for the analysis of chlortetracacline (CTC) in animal feed, a problem arose with the presence of peaks which did not return to baseline before the occurrence of a subsequent peak. Two methods were used to determine the peak height. In the first method, a perpendicular was dropped from the maximum of the second peak. A linear function was then fitted to the descending slope on the first peak. The point of intersection was taken as the baseline point. In the second approach, a polynomial fit was used instead. Results show excellent agreement with analysis obtained by established methodology. This approach provides a practical method for determination of chlortetracycline in animal feeds allowing up to six samples to be analyzed within 8 hours. © 1987 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

publication date

  • December 1, 1987

published in

Digital Object Identifier (DOI)

start page

  • 291

end page

  • 294

volume

  • 24

issue

  • 1