Immuno-slot blot assay for detection of UVR-mediated DNA damage Article

Karbaschi, M, Brady, NJ, Evans, MD et al. (2012). Immuno-slot blot assay for detection of UVR-mediated DNA damage . Methods in molecular biology (Clifton, N.J.), 920 163-175. 10.1007/978-1-61779-998-3_12

cited authors

  • Karbaschi, M; Brady, NJ; Evans, MD; Cooke, MS

abstract

  • Solar ultraviolet radiation (UVR), through the formation of DNA photolesions, is the primary cause of most skin cancers. A better understanding of the mechanisms of UVR-induced DNA damage may help prevent skin cancer and this may be achieved using methods to quantify DNA damage. The immuno-slot blot (ISB) method is routinely used for detection and quantification of any heat-and alkali-stable DNA adducts for which a sufficiently specific monoclonal antibody is available. The main steps in ISB are fragmentation and denaturation of the DNA, immobilization of DNA to a nitrocellulose filter, incubation with primary antibody against a specific DNA adduct, incubation with an enzyme-linked secondary antibody and finally chemiluminescence detection and quantification of the DNA adducts. © 2012 Springer Science+Business Media New York.

authors

publication date

  • January 1, 2012

published in

Digital Object Identifier (DOI)

start page

  • 163

end page

  • 175

volume

  • 920