Fabrication of arrayed topographic substrates and their effects on the electrobiophysical properties of neuronal cells
Article
Wu, Z, Zhu, M, Kisaalita, WS et al. (2011). Fabrication of arrayed topographic substrates and their effects on the electrobiophysical properties of neuronal cells
. 24(4), 467-474. 10.3969/j.issn.1004-1699.2011.04.001
Wu, Z, Zhu, M, Kisaalita, WS et al. (2011). Fabrication of arrayed topographic substrates and their effects on the electrobiophysical properties of neuronal cells
. 24(4), 467-474. 10.3969/j.issn.1004-1699.2011.04.001
UV lithography,silicon etching and soft lithography were adopted to fabricate micropillar arrayed cell culture substrates. With this approach,poly(dimethylsiloxane) ( PDMS) topographic substrates with pillar featuring dimensions equal to or larger than 4 μm could be successfully fabricated, with a pillar height of 4 μm. The fabrication of substrates with a pillar feafuring dimension of 2 μm might represent the maximal fabrication capability of this method,as pillar lodging or missing could be found. With a simple tilting angle method,we fabricated a topographic substrate formed with a packed polystyrene microbead array. The arrayed beads could be as small as 2 μm in diameter or even in submicrometer range. Cell culture experiments showed that arrayed topographical substrates can promote adhesion or spreading, affecting resting membrane potential establishement and enhance the responsiveness of voltage-gated calcium channels of neuronal cells. The arrayed topographic substrates provide an efficient tool for engineering the microenvironments for sensor neuronal cells.
