Lsi-based amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured pc12 cells Conference

Abe, H, Ino, K, Li, CZ et al. (2015). Lsi-based amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured pc12 cells . 1598-1600.

cited authors

  • Abe, H; Ino, K; Li, CZ; Kanno, Y; Inoue, KY; Suda, A; Kunikata, R; Matsudaira, M; Takahashi, Y; Shiku, H; Matsue, T

authors

abstract

  • We report a large-scale integration (LSI) based amperometric chip device containing 400 sensors with a pitch of 250 μm and its applications for biological analysis of dopamine release from three-dimensional (3D) cultured cells using rat pheochromocytoma (PC12) cells as a neural cell model. The 3D cultured PC12 cells (PC12 spheroids) were stimulated with a high K+ solution to induce dopamine release. We showed clearly drug effects of dopaminergic drugs L-3,4-dihydroxyphenylalanine, (L-DOPA) and reserpine on dopamine release from PC12 spheroids. Our results indicate that the device is a useful tool for evaluating the effects of dopaminergic drugs.

publication date

  • January 1, 2015

start page

  • 1598

end page

  • 1600