Ras-Activated Endocytosis Is Mediated by the Rab5 Guanine Nucleotide Exchange Activity of RIN1 Article

Tall, GG, Barbieri, MA, Stahl, PD et al. (2001). Ras-Activated Endocytosis Is Mediated by the Rab5 Guanine Nucleotide Exchange Activity of RIN1 . DEVELOPMENTAL CELL, 1(1), 73-82. 10.1016/S1534-5807(01)00008-9

cited authors

  • Tall, GG; Barbieri, MA; Stahl, PD; Horazdovsky, BF

authors

abstract

  • RIN1 was originally identified by its ability to inhibit activated Ras and likely participates in multiple signaling pathways because it binds c-ABL and 14-3-3 proteins, in addition to Ras. RIN1 also contains a region homologous to the catalytic domain of Vps9p-like Rab guanine nucleotide exchange factors (GEFs). Here, we show that this region is necessary and sufficient for RIN1 interaction with the GDP-bound Rabs, Vps21p, and Rab5A. RIN1 is also shown to stimulate Rab5 guanine nucleotide exchange, Rab5A-dependent endosome fusion, and EGF receptor-mediated endocytosis. The stimulatory effect of RIN1 on all three of these processes is potentiated by activated Ras. We conclude that Ras-activated endocytosis is facilitated, in part, by the ability of Ras to directly regulate the Rab5 nucleotide exchange activity of RIN1. © 2001 Cell Press.

publication date

  • July 1, 2001

published in

Digital Object Identifier (DOI)

start page

  • 73

end page

  • 82

volume

  • 1

issue

  • 1