The use of an inverse design and optimization technique which was originally developed for an aerospace application is illustrated. This technique can be adapted to the bio-heat transfer equations in order to determine exterior cooling conditions which will produce maximum survival rates throughout a given container. The goal of this project is to illustrate that the inverse design technique can be very useful in designing cryopreservation protocols. Initially, one guesses the exterior ambient temperature, and this is adjusted iteratively during the optimization procedure in order to achieve specified cooling conditions throughout the container. This inverse design technique is demonstrated for a very simple application in order to prove the feasibility and applicability of the technique.
