This work demonstrates a xanthine oxidase (XO) based electrochemical biosensor for detection of xanthine, a metabolic product of purines in wounds. XO immobilized on a multiwalled carbon nanotube (MWNT) and gold (Au) nanoparticle matrix provided an electron transfer pathway to monitor enzymatic xanthine oxidation. This nanostructured matrix acted also as a catalyst, assisting detection of enzymatic byproduct, hydrogen peroxide. The results show the biosensor has the detection limit of 0.1 nM, and an enhanced performance over a broad linear concentration range of 0.1 nM-7.3 mM compared to MWNT or Au individually. Electrochemical results over a broad pH range depicted a correlation model of the sensor signal with healing through its stages as the pH shifts from alkaline to acidic as recovery progresses. This shows that the biosensor can be used to not only track healing progress but also continuously monitor complications with a thorough assessment of biochemical tissue recuperation.