Image-guided therapy using nanoparticles have been used for various therapeutic applications such as drug and gene delivery, hyperthermia, and photodynamic therapy. These image-guided approaches help to achieve more efficient treatments by visualizing biodistribution of therapeutic agents and quantifying them at targeted tissues. This allows not only monitoring of their accumulation at targeted sites, but also highly localized treatments by controlled activation of therapeutic agents at the desired tissues, when needed. Here, we summarize the imaging modalities used for image-guided therapies and discuss recent advances on nanoparticle-based image-guided therapies.