Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism Article

Petroianu, G, Szoke, E, Kalász, H et al. (2009). Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism . Open Medicinal Chemistry Journal, 3 1-7. 10.2174/1874104500903010001

cited authors

  • Petroianu, G; Szoke, E; Kalász, H; Szegi, P; Laufer, R; Benkco, B; Darvas, F; Tekes, K

abstract

  • Three major flavonoid chamomile components (quercetin, apigenin-7-O- glucoside and rutin) were subjected to oxidative metabolism by cytochrome P-450 of rat liver microsomal preparations. Changes over time in their respective concentrations were followed using reversed-phase HPLC with UV detection. No clean-up had to be applied as only the specific flavonoid had to be separated from the background components originating from the rat liver microsome. Neither the concentration of apigenin-7-O-glucoside nor that of the diglycoside rutin decreased during one hour of exposure to rat microsomal treatment. In contrast, the concentration of quercetin, a lipophilic aglycon, decreased. Our analytical HPLC results complement the in silico calculated lipophilicity (logP) of these compounds; the relatively high lipophilicity of quercetin appears to predispose it to oxidative metabolism in order to decrease its fat solubility. In contrast the much less lipophilic compounds apigenin-7-O-glucoside and rutin were resistant in vitro to microsomal treatment. © A.A. El Maghraby; Licensee Bentham Open.

authors

publication date

  • September 24, 2009

published in

Digital Object Identifier (DOI)

start page

  • 1

end page

  • 7

volume

  • 3